The Deep-Sea Polyextremophile Halobacteroides lacunaris TB21 Rough-Type LPS: Structure and Inhibitory Activity towards Toxic LPS

被引：24

作者：

Di Lorenzo, Flaviana ^{[1
]}

Palmigiano, Angelo ^{[2
]}

Paciello, Ida ^{[3
]}

Pallach, Mateusz ^{[1
]}

Garozzo, Domenico ^{[2
]}

Bernardini, Maria-Lina ^{[3
]}

La Cono, Violetta ^{[4
]}

Yakimov, Michail M. ^{[4
,5
]}

Molinaro, Antonio ^{[1
]}

Silipo, Alba ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Chem Sci, I-80126 Naples, Italy

[2] CNR, IPCB, Unit Catania, I-95126 Catania, Italy

[3] Sapienza Univ Rome, Dept Biol & Biotechnol Charles Darwin, I-00185 Rome, Italy

[4] CNR, Inst Coastal Marine Environm, Marine Mol Microbiol Biotechnol, I-98122 Messina, Italy

[5] Immanuel Kant Balt Fed Univ, Kaliningrad 236040, Russia

来源：

MARINE DRUGS | 2017年 / 15卷 / 07期

基金：

欧盟地平线“2020”;

关键词：

extremophiles; halophiles; Halobacteroides; deep-sea hypersaline anoxic basins (DHABs); lipopolysaccharide (LPS); innate immunity; lipid A; core oligosaccharide; INNATE IMMUNITY; LIPID-A; BACTERIAL LIPOPOLYSACCHARIDES; ABSOLUTE-CONFIGURATION; STRUCTURE ELUCIDATION; OLIGOSACCHARIDE; RECOGNITION; ACTIVATION; SPECTRA; KDO;

D O I：

10.3390/md15070201

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The structural characterization of the lipopolysaccharide (LPS) from extremophiles has important implications in several biomedical and therapeutic applications. The polyextremophile Gram-negative bacterium Halobacteroides lacunaris TB21, isolated from one of the most extreme habitats on our planet, the deep-sea hypersaline anoxic basin Thetis, represents a fascinating microorganism to investigate in terms of its LPS component. Here we report the elucidation of the full structure of the R-type LPS isolated from H. lacunaris TB21 that was attained through a multi-technique approach comprising chemical analyses, NMR spectroscopy, and Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry. Furthermore, cellular immunology studies were executed on the pure R-LPS revealing a very interesting effect on human innate immunity as an inhibitor of the toxic Escherichia coli LPS.

引用

页数：16

共 38 条

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