Overproduction of geranylgeraniol in Coprinopsis cinerea by the expression of geranylgeranyl diphosphate synthase gene

被引：7

作者：

You, Lin-Feng ^{[1
]}

Guo, Li-Qiong ^{[1
]}

Lin, Jun-Fang ^{[1
]}

Ren, Tao ^{[2
]}

Wang, Jian-Rong ^{[1
]}

机构：

[1] South China Agr Univ, Dept Bioengn, Coll Food Sci, Guangzhou 510640, Guangdong, Peoples R China

[2] Harvard Univ, Sch Med, Dept Microbiol & Immunobiol, Boston, MA USA

来源：

JOURNAL OF BASIC MICROBIOLOGY | 2014年 / 54卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Coprinopsis cinerea; Ergosterol; Geranylgeraniol; Geranylgeranyl diphosphate synthase; Squalene; COPRINUS-CINEREUS; SESQUITERPENE SYNTHASES; TAXOL BIOSYNTHESIS; MOLECULAR-GENETICS; ESCHERICHIA-COLI; FUNGI; RECOMBINANT; SQUALENE;

D O I：

10.1002/jobm.201400152

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

(E, E, E)-Geranylgeraniol (GGOH) is a valuable ingredient of many perfumes and a valuable precursor for synthesizing pharmaceuticals. In an attempt to increase the GGOH concentration in Coprinopsis cinerea, we demonstrated that the expression of geranylgeranyl diphosphate synthase (ggpps) gene isolated from Taxus x media could promote GGOH production. Furthermore, the concentrations of squalene and ergosterol were measured in the engineered strains. Expectedly, significant decreases of squalene and ergosterol levels were observed in those strains transformed with ggpps gene. This could be explained by the partial redirection of metabolic flux from squalene to GGOH, whose biosynthesis competes for the same precursor with squalene. This work suggested that the expression of ggpps in higher fungi was an effective method for bio-production of GGOH.

引用

页码：1387 / 1394

页数：8

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