A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase

被引：15

作者：

Tada, Tatsuya ^{[1
]}

Miyoshi-Akiyama, Tohru ^{[1
,2
]}

Shimada, Kayo ^{[1
]}

Shiroma, Akino ^{[3
]}

Nakano, Kazuma ^{[3
]}

Teruya, Kuniko ^{[3
]}

Satou, Kazuhito ^{[3
]}

Hirano, Takashi ^{[3
]}

Shimojima, Masahiro ^{[4
]}

Kirikae, Teruo ^{[1
]}

机构：

[1] Natl Ctr Global Hlth & Med, Res Inst, Dept Infect Dis, Shinjuku Ku, 1-21-1 Toyama, Tokyo, Japan

[2] Natl Ctr Global Hlth & Med, Res Inst, Pathogen Microbe Lab, Shinjuku Ku, 1-21-1 Toyama, Tokyo, Japan

[3] Okinawa Inst Adv Sci, Div Res & Dev, Okinawa, Japan

[4] BML Inc, Dept Accuracy Management, Kawagoe, Saitama, Japan

来源：

PLOS ONE | 2016年 / 11卷 / 04期

关键词：

II TOPOISOMERASE MUTATIONS; GENE; STRAINS;

D O I：

10.1371/journal.pone.0149385

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-beta-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of bla(IMP-34), located at different sites on the chromosome. Each blaIMP-34 was present in the same structures of the class 1 integrons, tnpA(ISPa7)-intI1-qacG-bla(IMP-34)-aac(6')-Ib-qacEdelta1-sul1-orf5-tniBdelta-tniA. The isolate belonged to multilocus sequence typing ST235, one of the international high-risk clones. IMP-34, with an amino acid substitution (Glu126Gly) compared with IMP-1, hydrolyzed all beta-lactamases tested except aztreonam, and its catalytic activities were similar to IMP-1. This is the first report of a clinical isolate of an IMP-34-producing P. aeruginosa harboring two copies of blaI(MP-34) on its chromosome.

引用

页数：10

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