Fibroblast growth factor 21 regulates lipid accumulation and adipogenesis in goat intramuscular adipocyte

被引：20
作者：
Xu, Qing [1 ,2 ]
Lin, Sen [1 ,2 ]
Li, Qian [1 ,2 ]
Lin, Yaqiu [1 ,2 ]
Xiong, Yan [1 ,2 ]
Zhu, Jiangjiang [2 ,3 ]
Wang, Yong [2 ,3 ]
机构：
[1] Southwest Minzu Univ, Sch Life Sci & Technol, Chengdu 610041, Sichuan, Peoples R China
[2] Southwest Minzu Univ, Key Lab Qinghai Tibetan Plateau Anim Genet Resour, Minist Educ, Chengdu 610041, Sichuan, Peoples R China
[3] Qinghai Tibetan Plateau Anim Genet Resource Reser, Chengdu 610041, Sichuan, Peoples R China
来源：
ANIMAL BIOTECHNOLOGY | 2021年 / 32卷 / 03期
基金：
国家重点研发计划;
关键词：
FGF21; intramuscular adipocyte; differentiation; KLFs; ACTIVATED-RECEPTOR-GAMMA; TRANSCRIPTION FACTORS; GLUCOSE-HOMEOSTASIS; FACTOR GENE; CROSS-TALK; FGF21; EXPRESSION; OBESITY; FGF-21; FIBROBLAST-GROWTH-FACTOR-21;
D O I：
10.1080/10495398.2019.1691010
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Fibroblast growth factor 21 (FGF21) plays a critical role in the regulation of lipid metabolism; however, its function in goat intramuscular fat (IMF) deposition remains unknown. To explore the role of FGF21 for goat IMF deposition, we performed gain and loss function of FGF21 in intramuscular adipocyte. Our results showed that overexpression of FGF21 mediated by adenovirus inhibits lipid accumulation of goat intramuscular adipocyte, accompanied by down-regulating the mRNA levels of peroxisome proliferator activated receptor gamma (PPAR gamma), adipocyte fatty acid-binding protein 2 (aP2) and sterol regulatory element-binding protein 1 (SREBP1), but up-regulating counterpart of preadipocyte factor1 (Pref1). Conversely, siRNAs knocking down FGF21 promotes the expression of PPAR gamma and CCAAT/enhancer binding protein-alpha (C/EBP alpha) but suppressed that of lipoprotein lipase (LPL) and Pref1. Meanwhile, we found that FGF21 regulates the expression of many KLFs transcription factors, such as KLF3, 7, 9, 11, 14, and 16. These findings demonstrate a key role of FGF21 as a negative factor in the regulation of adipogenic differentiation in goat intramuscular preadipocyte.
引用
收藏
页码：318 / 326
页数：9
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