Reactivity and Selectivity Principles in Native Protein Bioconjugation

被引：12

作者：

Adakkattil, Ramesh ^{[1
]}

Thakur, Kalyani ^{[1
]}

Rai, Vishal ^{[1
]}

机构：

[1] Indian Inst Sci Educ & Res Bhopal, Dept Chem, Bhopal Bypass Rd, Bhopal 462066, Madhya Pradesh, India

来源：

CHEMICAL RECORD | 2021年 / 21卷 / 08期

关键词：

Chemoselectivity; Site-selectivity; Site-specificity; Bioconjugation; Protein-specificity; Antibody-drug conjugates; SITE-SPECIFIC CONJUGATION; NEXT-GENERATION; ONE-POT; DRUG; ANTIBODY; CHEMISTRY; STRATEGIES; PEPTIDES; THERAPEUTICS; ALKYLATION;

D O I：

10.1002/tcr.202100108

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Are chemical methods capable of precisely engineering the native proteins? Is it possible to develop platforms that can empower the regulation of chemoselectivity, site-selectivity, modularity, protein-specificity, and site-specificity? This account delineates our research journey in the last ten years on the developments revolving around these questions. It will range from the realization of chemoselective and site-selective labeling of reactivity hotspots to modular linchpin directed modification (LDM (R)) platform and site-specific Gly-tag (R) technology. Also, we outline a few biotechnology tools, including Maspecter (R), that accelerated the detailed analysis of the bioconjugates and rendered a powerful toolbox for homogeneous antibody-drug conjugates (ADCs).

引用

页码：1941 / 1956

页数：16

共 86 条

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