Utilization of the peroxidase-like activity of silver nanoparticles nanozyme on O-phenylenediamine/H2O2 system for fluorescence detection of mercury (II) ions

被引：29

作者：

Abdel-Lateef, Mohamed A. ^{[1
]}

机构：

[1] Al Azhar Univ, Fac Pharm, Dept Pharmaceut Analyt Chem, Assiut Branch, Assiut 71524, Egypt

来源：

SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期

关键词：

SELECTIVE DETECTION; COLORIMETRIC SENSOR; HYDROGEN-PEROXIDE; WATER; GOLD; SIZE; OXIDATION; DEGRADATION; PLATINUM; SHAPE;

D O I：

10.1038/s41598-022-10779-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Polyvinylpyrrolidone stabilized silver nanoparticles (PV-AgNPs) were synthesized from AgNO3/trisodium citrate and with the assistance of microwave energy. The synthesized PV-AgNPs were found to own an actual peroxidase mimicking activity. This catalytic activity can oxidize the non-fluorescence reagent (o-phenylenediamine) to a high fluorescence reaction product (2,3-diaminophenazine). The reaction product exhibited a fluorescence emission at 563 nm upon the excitation at 420. Among many metals, only mercury (II) ions can inhibit the catalytic activity of PV-AgNPs nanozyme. Accordingly, the fluorescence intensity of the reaction product has been successfully quenched. This quenching effect in the fluorescence intensity was directly proportional to the concentration of mercury (II). Depending on this finding, a simple, cost-effective, and selective spectrofluorimetric approach has been designed for mercury (II) detection in water samples. The linear relationship between the inhibition in fluorescence intensity and mercury (II) concentration was found in 20-2000 nM with a detection limit of 8.9 nM.

引用

页数：9

共 43 条

[1] Spectrofluorimetric determination of α-difluoromethylornithine through condensation with ninhydrin and phenylacetaldehyde: application to pharmaceutical cream and spiked urine samples [J].