Optical Thermophoresis for Quantifying the Buffer Dependence of Aptamer Binding

被引：174

作者：

Baaske, Philipp ^{[1
,2
]}

Wienken, Christoph J. ^{[1
]}

Reineck, Philipp ^{[1
]}

Duhr, Stefan ^{[2
]}

Braun, Dieter ^{[1
]}

机构：

[1] Univ Munich, Dept Phys, Ctr NanoSci CeNS, D-80799 Munich, Germany

[2] NanoTemper Technol GmbH, D-80799 Munich, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 12期

关键词：

aptamers; DNA; drug discovery; immobilization-free methods; proteins; SINGLE-STRANDED-DNA; MOLECULAR RECOGNITION; CHARGED COLLOIDS; SELECTION; THERMODIFFUSION; FLUORESCENCE; DIFFUSION; ADENOSINE; INVITRO; LIGANDS;

D O I：

10.1002/anie.200903998

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

"Chemical Equation Presented" Some like it hot: A robust and fast method for characterizing aptamers relies on the distinct thermophoretic movements of molecules in microscopic temperature gradients (see diagram). The binding properties of proteins and even small molecules can be measured within seconds, and less than 1 μL of sample is required. Notably, the technique works well in complex liquids such as human serum. © 2010 Wiley-VCH Verlag GmbH & Co. KCaA.

引用

页码：2238 / 2241

页数：4

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