Measurement of serum nitrite/nitrate concentrations using high-performance liquid chromatography

被引：35

作者：

El Menyawi, I

Looareesuwan, S

Knapp, S

Thalhammer, F

Stoiser, B

Burgmann, H

机构：

[1] Univ Vienna, Div Infect Dis, Dept Internal Med 1, A-1090 Vienna, Austria

[2] Mahidol Univ, Fac Trop Med, Dept Clin Trop Med, Bangkok, Thailand

[3] Univ Vienna, Dept Internal Med 1, Intens Care Unit 1312, Vienna, Austria

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1998年 / 706卷 / 02期

关键词：

nitrite; nitrate; nitric oxide;

D O I：

10.1016/S0378-4347(97)00570-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have reported increased serum concentrations of nitrite/nitrate - the degradation products of nitric oxide - in Plasmodium vivax malaria and uncomplicated Plasmodium falciparum um malaria, In all these studies, however, nitrite/nitrate has been measured spectrometrically using Griess reagent which carries major disadvantages in the determination of serum nitrite/nitrate. The method does not allow an exact differentiation of nitrite and biogenic amines that are physiologically present in plasma. In the present study we introduce high-performance liquid chromatography as a new, accurate and cost effective method for determination of serum nitrite/nitrate levels. Significantly increased nitrate concentrations were found in malaria patients and serum values remained above normal levels for at least 21 days. It could be shown that our HPLC method is a sensitive and cost-effective method for direct determination of nitrite/nitrate in serum samples, which is not influenced by the presence of biogenic amines. (C) 1998 Elsevier Science B.V.

引用

页码：347 / 351

页数：5

共 11 条

[1] POSSIBLE CENTRAL ROLE OF NITRIC-OXIDE IN CONDITIONS CLINICALLY SIMILAR TO CEREBRAL MALARIA
CLARK, IA
ROCKETT, KA
COWDEN, WB
[J]. LANCET, 1992, 340 (8824) : 894 - 896
[2] NITRIC-OXIDE IN CEREBRAL MALARIA
COT, S
RINGWALD, P
MULDER, B
MIAILHES, P
YAPYAP, J
NUSSLER, AK
ELING, WMC
[J]. JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (06) : 1417 - 1418
[3] FRITZ JS, 1982, ION CHROMATOGRAPHY, P102
[4] NITRO CONTAINING L-ARGININE ANALOGS INTERFERE WITH ASSAYS FOR NITRATE AND NITRITE
GREENBERG, SS
XIE, JM
SPITZER, JJ
WANG, JF
LANCASTER, J
GRISHAM, MB
POWERS, DR
GILES, TD
[J]. LIFE SCIENCES, 1995, 57 (21) : 1949 - 1961
[5] JACOBS P, 1995, J IMMUNOL, V155, P5306
[6] High plasma levels of nitrogen oxides are associated with severe disease and correlate with rapid parasitological and clinical cure in Plasmodium falciparum malaria
Kremsner, PG
Winkler, S
Wildling, E
Prada, J
Bienzle, U
Graninger, W
Nussler, AK
[J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1996, 90 (01) : 44 - 47
[7] NITRIC-OXIDE IN INFLAMMATION AND IMMUNE-RESPONSE
MOILANEN, E
VAPAATALO, H
[J]. ANNALS OF MEDICINE, 1995, 27 (03) : 359 - 367
[8] NITRIC-OXIDE RELEASE ACCOUNTS FOR THE BIOLOGICAL-ACTIVITY OF ENDOTHELIUM-DERIVED RELAXING FACTOR
PALMER, RMJ
FERRIGE, AG
MONCADA, S
[J]. NATURE, 1987, 327 (6122) : 524 - 526
[9] KILLING OF PLASMODIUM-FALCIPARUM INVITRO BY NITRIC-OXIDE DERIVATIVES
ROCKETT, KA
AWBURN, MM
COWDEN, WB
CLARK, IA
[J]. INFECTION AND IMMUNITY, 1991, 59 (09) : 3280 - 3283
[10] SCHMIDT HHHW, 1992, MOL PHARMACOL, V41, P615

← 1 2 →