A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

被引：42
作者：
Shi, Gongyao [1 ,2 ]
Hao, Mengyuan [1 ,2 ]
Tian, Baoming [1 ,3 ]
Cao, Gangqiang [1 ,3 ]
Wei, Fang [1 ,3 ]
Xie, Zhengqing [1 ,3 ]
机构：
[1] Zhengzhou Univ, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou, Peoples R China
[2] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Peoples R China
[3] Zhengzhou Univ, Sch Agr Sci, Henan Int Joint Lab Crop Gene Resources & Improve, Zhengzhou, Peoples R China
来源：
FRONTIERS IN PLANT SCIENCE | 2021年 / 12卷
基金：
中国国家自然科学基金;
关键词：
TRV-VIGS; vector construction; agroinfiltration; secondary inoculation; methodology modification; RESISTANCE SIGNALING PATHWAYS; N-MEDIATED RESISTANCE; STRIPE-MOSAIC-VIRUS; TRANSCRIPTION FACTORS; LOW-TEMPERATURE; PROTEIN; DEFENSE; VECTOR; VIGS; EFFICIENT;
D O I：
10.3389/fpls.2021.671091
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.
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页数：14
相关论文
共 96 条
  • [51] Virus-induced gene silencing in tomato
    Liu, YL
    Schiff, M
    Dinesh-Kumar, SP
    [J]. PLANT JOURNAL, 2002, 31 (06) : 777 - 786
  • [52] Involvement of MEK1 MAPKK, NTF6 MAPK, WRKY/MYB transcription factors, COI1 and CTR1 in N-mediated resistance to tobacco mosaic virus
    Liu, YL
    Schiff, M
    Dinesh-Kumar, SP
    [J]. PLANT JOURNAL, 2004, 38 (05) : 800 - 809
  • [53] Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus
    Liu, YL
    Schiff, M
    Marathe, R
    Dinesh-Kumar, SP
    [J]. PLANT JOURNAL, 2002, 30 (04) : 415 - 429
  • [54] Lu L.L., 2011, NO HORTICULTURE, V16, P139
  • [55] High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance
    Lu, R
    Malcuit, I
    Moffett, P
    Ruiz, MT
    Peart, J
    Wu, AJ
    Rathjen, JP
    Bendahmane, A
    Day, L
    Baulcombe, DC
    [J]. EMBO JOURNAL, 2003, 22 (21) : 5690 - 5699
  • [56] Misra RC, 2020, METHODS MOL BIOL, V2172, P123, DOI 10.1007/978-1-0716-0751-0_10
  • [57] Virus-induced Gene Silencing in Streptocarpus rexii (Gesneriaceae)
    Nishii, Kanae
    Fei, Yue
    Hudson, Andrew
    Moller, Michael
    Molnar, Attila
    [J]. MOLECULAR BIOTECHNOLOGY, 2020, 62 (6-7) : 317 - 325
  • [58] Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit
    Orzaez, D
    Mirabel, S
    Wieland, WH
    Granell, A
    [J]. PLANT PHYSIOLOGY, 2006, 140 (01) : 3 - 11
  • [59] Palmer L, 2020, METHODS MOL BIOL, V2172, P111, DOI 10.1007/978-1-0716-0751-0_9
  • [60] Virus-induced gene silencing: A versatile tool for discovery of gene functions in plants
    Purkayastha, Arunima
    Dasgupta, Indranil
    [J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2009, 47 (11-12) : 967 - 976
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