A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

被引：42
作者：
Shi, Gongyao [1 ,2 ]
Hao, Mengyuan [1 ,2 ]
Tian, Baoming [1 ,3 ]
Cao, Gangqiang [1 ,3 ]
Wei, Fang [1 ,3 ]
Xie, Zhengqing [1 ,3 ]
机构：
[1] Zhengzhou Univ, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou, Peoples R China
[2] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Peoples R China
[3] Zhengzhou Univ, Sch Agr Sci, Henan Int Joint Lab Crop Gene Resources & Improve, Zhengzhou, Peoples R China
来源：
FRONTIERS IN PLANT SCIENCE | 2021年 / 12卷
基金：
中国国家自然科学基金;
关键词：
TRV-VIGS; vector construction; agroinfiltration; secondary inoculation; methodology modification; RESISTANCE SIGNALING PATHWAYS; N-MEDIATED RESISTANCE; STRIPE-MOSAIC-VIRUS; TRANSCRIPTION FACTORS; LOW-TEMPERATURE; PROTEIN; DEFENSE; VECTOR; VIGS; EFFICIENT;
D O I：
10.3389/fpls.2021.671091
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.
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页数：14
相关论文
共 96 条
  • [91] Vacuum and Co-cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize
    Zhang, Ju
    Yu, Deshui
    Zhang, Yi
    Liu, Kun
    Xu, Kedong
    Zhang, Fuli
    Wang, Jian
    Tan, Guangxuan
    Nie, Xianhui
    Ji, Qiaohua
    Zhao, Lu
    Li, Chengwei
    [J]. FRONTIERS IN PLANT SCIENCE, 2017, 8
  • [92] [张晓萝 Zhang Xiaoluo], 2014, [华北农学报, Acta Agriculturae Boreali-Sinica], V29, P36
  • [93] Expression of RPS4 in tobacco induces an AvrRps4-independent HR that requires EDS1, SGT1 and HSP90
    Zhang, Y
    Dorey, S
    Swiderski, M
    Jones, JDG
    [J]. PLANT JOURNAL, 2004, 40 (02) : 213 - 224
  • [94] Zhao JP, 2020, METHODS MOL BIOL, V2172, P39, DOI 10.1007/978-1-0716-0751-0_4
  • [95] Elucidating the role of highly homologous Nicotiana benthamiana ubiquitin E2 gene family members in plant immunity through an improved virus-induced gene silencing approach
    Zhou, Bangjun
    Zeng, Lirong
    [J]. PLANT METHODS, 2017, 13
  • [96] The Feasibility Analysis of PVX and TRV Vectors as the VIGS Tool for Studying the Gene Function
    Zhou Xiao-fu
    Sun Jing-di
    Zhao Zhuo
    Lv Jie
    Wei Xiao-wei
    Cai Rui
    Xu Hong-wei
    [J]. 2012 INTERNATIONAL CONFERENCE ON MEDICAL PHYSICS AND BIOMEDICAL ENGINEERING (ICMPBE2012), 2012, 33 : 46 - 54
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