A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

被引：42
作者：
Shi, Gongyao [1 ,2 ]
Hao, Mengyuan [1 ,2 ]
Tian, Baoming [1 ,3 ]
Cao, Gangqiang [1 ,3 ]
Wei, Fang [1 ,3 ]
Xie, Zhengqing [1 ,3 ]
机构：
[1] Zhengzhou Univ, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou, Peoples R China
[2] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Peoples R China
[3] Zhengzhou Univ, Sch Agr Sci, Henan Int Joint Lab Crop Gene Resources & Improve, Zhengzhou, Peoples R China
来源：
FRONTIERS IN PLANT SCIENCE | 2021年 / 12卷
基金：
中国国家自然科学基金;
关键词：
TRV-VIGS; vector construction; agroinfiltration; secondary inoculation; methodology modification; RESISTANCE SIGNALING PATHWAYS; N-MEDIATED RESISTANCE; STRIPE-MOSAIC-VIRUS; TRANSCRIPTION FACTORS; LOW-TEMPERATURE; PROTEIN; DEFENSE; VECTOR; VIGS; EFFICIENT;
D O I：
10.3389/fpls.2021.671091
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.
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页数：14
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