In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system

被引：18

作者：

Afonso, Tiago Barros ^{[1
]}

Simoes, Lucia Chaves ^{[1
]}

Lima, Nelson ^{[1
]}

机构：

[1] Univ Minho, Ctr Biol Engn, CEB, Braga, Portugal

来源：

BIOFOULING | 2019年 / 35卷 / 10期

关键词：

Penicillium expansum; Penicillium brevicompactum; bacteria; biomass quantification; microtitre plates; microscopy; MICROBIAL ECOLOGY; CORROSION; FUSARIUM; MOLDS; IRON;

D O I：

10.1080/08927014.2019.1688793

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The main focus so far in the study of biofilm formation in drinking water has been bacteria. Studies on biofilm formation involving filamentous fungi are, therefore, scarce. This study aimed to assess and characterize the ability of these microorganisms to interact with bacteria whilst forming inter-kingdom biofilms. Biofilms were analysed in terms of total biomass, metabolic activity, bacterial colony forming units and morphology by epifluorescence microscopy. The quantitative methods revealed that biofilm mass increased over time for both single and inter-kingdom biofilms, while specific metabolic activity decreased, in general, along the time points evaluated. Microscopic data visually confirmed the biofilm mass increase over time. This study shows that fungal stage development is important in the first 24 h of biofilm formation. Inter-kingdom biofilm formation is microorganism dependent and inter-kingdom biofilms may provide an advantage to the opportunistic bacterium Acinetobacter calcoaceticus to replicate and proliferate when compared with Methylobacterium oryzae.

引用

页码：1041 / 1054

页数：14

共 59 条

[1]

Ahmad I., 2012, CURRENT MICROSCOPY C, P646

[2] Pathogenic molds (including Aspergillus species) in hospital water distribution systems:: a 3-year prospective study and clinical implications for patients with hematologic malignancies [J].