Guidance for fluorescence in situ hybridization testing in hematologic disorders

被引:100
作者
Wolff, Daynna J.
Bagg, Adam
Cooley, Linda D.
Dewald, Gordon W.
Hirsch, Betsy A.
Jacky, Peter B.
Rao, Kathleen W.
Rao, P. Nagesh
机构
[1] Assoc Mol Pathol, Bethesda, MD 20814 USA
[2] Med Univ S Carolina, Dept Pathol & Lab Med, Charleston, SC 29425 USA
[3] Univ Penn, Sch Med, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA
[4] Mayo Clin, Dept Lab Med & Pathol, Rochester, MN USA
[5] Childrens Mercy Hosp, Pediat Sect Med Genet, Kansas City, KS USA
[6] Univ Min & Met Leoben, Sch Med, Dept Lab Med & Pathol, A-8700 Leoben, Austria
[7] NW Permanente, Dept Pathol Genet, Portland, OR USA
[8] Univ N Carolina, Dept Pediat, Chapel Hill, NC 27514 USA
[9] Univ Calif Los Angeles, Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90024 USA
关键词
D O I
10.2353/jmoldx.2007.060128
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Fluorescence in situ hybridization (FISH) provides an important adjunct to conventional cytogenetics and molecular studies in the evaluation of chromosome abnormalities associated with hematologic malignancies. FISH employs DNA probes and methods that are generally not Food and Drug Administration-approved, and therefore, their use as analyte-specific reagents involves unique pre- and postanalytical requirements. We provide an overview of the technical parameters influencing a reliable FISH, result and encourage laboratories to adopt specific procedures and policies in implementing metaphase and interphase FISH testing. A rigorous technologist training program relative to specific types of probes is detailed, as well as guidance for consistent interpretation of findings, including typical and atypical abnormal results. Details are provided on commonly used dual-fusion, extra signal, and break-apart probes, correct FISH nomenclature in the reporting of results, and the use of FISH in relation to other laboratory testing in the ongoing monitoring of disease. This article provides laboratory directors detailed guidance to he used in conjunction with existing regulations to successfully implement a FISH testing program or to assess current practices, allowing for optimal clinical testing for patient care.
引用
收藏
页码:134 / 143
页数:10
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