Quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets

被引:192
作者
Wise, M. G. [1 ]
Siragusa, G. R. [1 ]
机构
[1] USDA ARS, Russell Res Ctr, Poultry Microbiol Safety Res Unit, Athens, GA 30605 USA
关键词
antibiotic growth promotants; broiler chickens; gastrointestinal tract microbial ecology; gut microflora; quantitative real-time PCR; 16S rDNA;
D O I
10.1111/j.1365-2672.2006.03153.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic growth promotants (AGPs) on a vegetable-based diet. Methods and Results: Quantitative, real-time PCR with group-specific 16S rDNA primer sets was used to enumerate the abundance of the following chicken gastrointestinal (GI) tract phylogenetic groups: the Clostridium leptum-Faecalibacterium prausnitzii subgroup (Clostridium genus cluster IV), the Clostridium coccoides-Eubacterium rectale subgroup (Clostridium cluster XIVa and XIVb), the Bacteroides group (including Prevotella and Porphyromonas), Bifidobacterium spp., the Enterobacteriaceae, the Lactobacillus group (including the genera Leuconostoc, Pediococcus, Aerococcus and Weissella), the Clostridium perfringens subgroup (Clostridium cluster I), Enterococcus spp., Veillonella spp., Atopobium spp., Campylobacter spp. and the domain Bacteria. A species-specific 5'-nuclease (Taqman) assay was also employed to specifically assess Cl. perfringens abundance. Ten birds were sampled from each of two commercial chicken houses, one in which feed was supplemented with AGPs and exogenous animal protein, and the other vegetable-based and drug-free, at 7, 14 and 21 days of age. The ileal community was dominated by two large populations, the lactobacilli and the Enterobacteriaceae, with those taxa much more numerous in drug-free vegetable-based diet fed birds than those conventionally reared at the 7- and 14-day time periods. The progressive changes in microflora in both the conventional and drug-free caeca were similar to each other, with the Enterobacteriaceae sequences dominating at day 7, but being replaced by obligate anaerobe signature sequences by day 14. Of note was the finding that all the day 14 and day 21 replicate caecal samples from the drug-free house were positive for Campylobacter spp. averaging > 10(8) 16S rDNA gene copies per gram wet weight. Conclusions: Quantitative, real-time PCR indicates that the effects of drug-free rearing on the chicken GI tract microbial community are most pronounced in the ileal region, but AGPs may be important in controlling Campylobacter colonization of the caecum. Significance and Impact of the Study: A quantitative taxonomic understanding of the shifting microbial ecology of the broiler chicken gut microbiota is important in the light of AGP withdrawal. AGP withdrawal has occurred in response to concerns over the transfer of antimicrobial-resistant bacteria to humans via the food production chain.
引用
收藏
页码:1138 / 1149
页数:12
相关论文
共 60 条
[1]   INTERACTION OF CLOSTRIDIUM-PERFRINGENS AND ITS TOXINS IN PRODUCTION OF NECROTIC ENTERITIS OF CHICKENS [J].
ALSHEIKHLY, F ;
TRUSCOTT, RB .
AVIAN DISEASES, 1977, 21 (02) :256-263
[2]   PATHOLOGY OF NECROTIC ENTERITIS OF CHICKENS FOLLOWING INFUSION OF BROTH CULTURES OF CLOSTRIDIUM-PERFRINGENS INTO DUODENUM [J].
ALSHEIKHLY, F ;
TRUSCOTT, RB .
AVIAN DISEASES, 1977, 21 (02) :230-240
[3]   Microflora ecology of the chicken intestine using 16S ribosomal DNA primers [J].
Amit-Romach, E ;
Sklan, D ;
Uni, Z .
POULTRY SCIENCE, 2004, 83 (07) :1093-1098
[4]  
Apajalahti JHA, 1998, APPL ENVIRON MICROB, V64, P4084
[5]   Percent G+C profiling accurately reveals diet-related differences in the gastrointestinal microbial community of broiler chickens [J].
Apajalahti, JHA ;
Kettunen, A ;
Bedford, MR ;
Holben, WE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (12) :5656-5667
[6]   INTESTINAL MICROFLORA OF POULTRY AND GAME BIRDS DURING LIFE AND AFTER STORAGE [J].
BARNES, EM .
JOURNAL OF APPLIED BACTERIOLOGY, 1979, 46 (03) :407-+
[7]  
BARNES EM, 1972, AM J CLIN NUTR, V25, P1475
[8]   INTESTINAL FLORA OF CHICKEN IN PERIOD 2 TO 6 WEEKS OF AGE, WITH PARTICULAR REFERENCE TO ANAEROBIC BACTERIA [J].
BARNES, EM ;
BARNUM, DA ;
HARRY, EG ;
MEAD, GC .
BRITISH POULTRY SCIENCE, 1972, 13 (03) :311-&
[9]   Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota [J].
Bartosch, S ;
Fite, A ;
Macfarlane, GT ;
McMurdo, MET .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (06) :3575-3581
[10]   EVIDENCE FOR AN ANIMAL ORIGIN OF VANCOMYCIN-RESISTANT ENTEROCOCCI [J].
BATES, J ;
JORDENS, Z ;
SELKON, JB .
LANCET, 1993, 342 (8869) :490-491