Inactivation of classical swine fever virus: association of hydrostatic pressure and ultraviolet irradiation

被引：13

作者：

Freitas, TRP

Gaspar, LP

Caldas, LA

Silva, JL

Rebello, MA ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Dept Virol, BR-21941590 Rio De Janeiro, Brazil

[2] Inst Ciencias Biomed, Dept Bioquim Med, Rio De Janeiro, Brazil

来源：

JOURNAL OF VIROLOGICAL METHODS | 2003年 / 108卷 / 02期

关键词：

classical swine fever virus; inactivation; high hydrostatic pressure; ultraviolet irradiation;

D O I：

10.1016/S0166-0934(02)00289-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reversible pressure-induced disassembly of several viruses has suggested the idea of using hydrostatic pressure to suppress virus infectivity. In this study, the effects of high hydrostatic pressure and ultraviolet (UV) irradiation were investigated on classical swine fever virus (CSFV) in an attempt to eliminate residual infectivity. The structural modifications were followed by intrinsic fluorescence and biological activity assays. The kinetics of CSFV inactivation showed that pressure-induced inactivation was not enough to eliminate viral infectivity. However, when pressure was applied in association with UV irradiation no infectious focus was observed. The application of these two methods against CSFV can be an attractive inactivation strategy for the development of a vaccine. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：205 / 211

页数：7

共 44 条

[1] Pressure cycling technology: a novel approach to virus inactivation in plasma [J].

Bradley, DW ;

Hess, RA ;

Tao, F ;

Sciaba-Lentz, L ;

Remaley, AT ;

Laugharn, JA ;

Manak, M .

TRANSFUSION, 2000, 40 (02) :193-200

[2] PRINCIPLES OF SELECTIVE INACTIVATION OF VIRAL GENOME .1. UV-INDUCED INACTIVATION OF INFLUENZA-VIRUS [J].

BUDOWSKY, EI ;

BRESLER, SE ;

FRIEDMAN, EA ;

ZHELEZNOVA, NV .

ARCHIVES OF VIROLOGY, 1981, 68 (3-4) :239-247

[3] Screening of protective antigens of Japanese encephalitis virus by DNA immunization: a comparative study with conventional viral vaccines [J].

Chen, HW ;

Pan, CH ;

Liau, MY ;

Jou, RW ;

Tsai, CJ ;

Wu, HJ ;

Lin, YL ;

Tao, MH .

JOURNAL OF VIROLOGY, 1999, 73 (12) :10137-10145

[4] REVERSIBLE PRESSURE DISSOCIATION OF R17 BACTERIOPHAGE - THE PHYSICAL INDIVIDUALITY OF VIRUS-PARTICLES [J].

DAPOIAN, AT ;

OLIVEIRA, AC ;

GASPAR, LP ;

SILVA, JL ;

WEBER, G .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (04) :999-1008

[5] EQUINE ARTERITIS VIRUS SUBGENOMIC RNA-TRANSCRIPTION - UV INACTIVATION AND TRANSLATION INHIBITION STUDIES [J].

DENBOON, JA ;

SPAAN, WJM ;

SNIJDER, EJ .

VIROLOGY, 1995, 213 (02) :364-372

[6]

Freitas T. R. P., 1999, Revista Brasileira de Medicina Veterinaria, V21, P126

[7] Partially folded states of the capsid protein of cowpea severe mosaic virus in the disassembly pathway [J].

Gaspar, LP ;

Johnson, JE ;

Silva, JL ;

DaPoian, AT .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (02) :456-466

[8] Hydrostatic pressure induces the fusion-active state of enveloped viruses [J].

Gaspar, LP ;

Silva, ACB ;

Gomes, AMO ;

Freitas, MS ;

Bom, APDA ;

Schwarcz, WD ;

Mestecky, J ;

Novak, MJ ;

Foguel, D ;

Silva, JL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (10) :8433-8439

[9] The metastable state of nucleocapsids of enveloped viruses as probed by high hydrostatic pressure [J].

Gaspar, LP ;

Terezan, AF ;

Pinheiro, AS ;

Foguel, D ;

Rebello, MA ;

Silva, JL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (10) :7415-7421

[10] ENHANCEMENT OF PROTECTIVE IMMUNE-RESPONSES TO VENEZUELAN EQUINE ENCEPHALITIS (VEE) VIRUS WITH MICROENCAPSULATED VACCINE [J].

GREENWAY, TE ;

ELDRIDGE, JH ;

LUDWIG, G ;

STAAS, JK ;

SMITH, JF ;

GILLEY, RM ;

MICHALEK, SM .

VACCINE, 1995, 13 (15) :1411-1420

← 1 2 3 4 5 →