In-situ Hybridization for the Detection of Sacbrood Virus in Infected Larvae of the Honey Bee (Apis cerana)

被引：5

作者：

Park, C. ^{[1
]}

Kang, H. S. ^{[1
]}

Jeong, J. ^{[1
]}

Kang, I. ^{[1
]}

Choi, K. ^{[1
]}

Yoo, M. -S. ^{[2
]}

Kim, Y. -H. ^{[2
]}

Kang, S. -W. ^{[2
]}

Lim, H. -Y. ^{[3
]}

Yoon, B. -S. ^{[3
]}

Chae, C. ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Vet Med, Dept Vet Pathol, 1 Gwanak Ro, Seoul, South Korea

[2] Natl Vet Res Quarantine Serv, 480 Anyang 6 Dong, Anyang, South Korea

[3] Kyonggi Univ, Coll Nat Sci, Dept Life Sci, Suwon, South Korea

来源：

JOURNAL OF COMPARATIVE PATHOLOGY | 2016年 / 154卷 / 2-3期

关键词：

Apis cerana; Iflavirus; in-situ hybridization; sacbrood virus; MELLIFERA;

D O I：

10.1016/j.jcpa.2015.12.003

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The aim of this study was to develop and use in-situ hybridization (ISH) for the detection and localization of the sacbrood virus (SBV) in Korean honey bee (Apis cerana) larvae that were infected naturally with SBV. A 258 base pair cDNA probe for SBV was generated by polymerase chain reaction. Cells positive for viral genome typically showed a dark brown reaction in the cytoplasm. SBV was detected consistently in trophocytes and urocytes. The ISH was successfully applied to routinely fixed and processed tissues and thus should prove helpful in the diagnosis and characterization of viral distribution in infected larvae. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：258 / 262

页数：5

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