Biomolecular interaction analysis: affinity biosensor technologies for functional analysis of proteins

被引：103
作者：
Malmqvist, M [1 ]
Karlsson, R [1 ]
机构：
[1] Biacore AB, S-75450 Uppsala, Sweden
来源：
CURRENT OPINION IN CHEMICAL BIOLOGY | 1997年 / 1卷 / 03期
关键词：
D O I：
10.1016/S1367-5931(97)80077-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The introdution of affinity-based biosensors has permitted label-free functional analysis of biomolecular interactions in real time. A variety of methods are now based on BIOCORE(registered trade-mark) and IAsys technology and have mainly been used to determine kinetics and affinity constants.
引用
收藏
页码：378 / 383
页数：6
相关论文
共 47 条
  • [1] Detection and characterization of oligosaccharides in column effluents using surface plasmon resonance
    Blikstad, I
    Fagerstam, LG
    Bhikhabhai, R
    Lindblom, H
    [J]. ANALYTICAL BIOCHEMISTRY, 1996, 233 (01) : 42 - 49
  • [2] Theoretical analysis of protein concentration determination using biosensor technology under conditions of partial mass transport limitation
    Christensen, LLH
    [J]. ANALYTICAL BIOCHEMISTRY, 1997, 249 (02) : 153 - 164
  • [3] COMPARISON OF A STRUCTURAL AND A FUNCTIONAL EPITOPE
    CUNNINGHAM, BC
    WELLS, JA
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1993, 234 (03) : 554 - 563
  • [4] The structure and ligand interactions of CD2: Implications for T-cell function
    Davis, SJ
    vanderMerwe, PA
    [J]. IMMUNOLOGY TODAY, 1996, 17 (04): : 177 - 187
  • [5] Fisher Robert J., 1994, Current Opinion in Biotechnology, V5, P389, DOI 10.1016/0958-1669(94)90047-7
  • [6] STABLE CHELATING LINKAGE FOR REVERSIBLE IMMOBILIZATION OF OLIGOHISTIDINE TAGGED PROTEINS IN THE BIACORE SURFACE-PLASMON RESONANCE DETECTOR
    GERSHON, PD
    KHILKO, S
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 183 (01) : 65 - 76
  • [7] ANTIGEN-ANTIBODY BINDING AND MASS-TRANSPORT BY CONVECTION AND DIFFUSION TO A SURFACE - A 2-DIMENSIONAL COMPUTER-MODEL OF BINDING AND DISSOCIATION KINETICS
    GLASER, RW
    [J]. ANALYTICAL BIOCHEMISTRY, 1993, 213 (01) : 152 - 161
  • [8] Goldbaum FA, 1996, J MOL RECOGNIT, V9, P6, DOI 10.1002/(SICI)1099-1352(199601)9:1<6::AID-JMR240>3.3.CO
  • [9] 2-M
  • [10] HUMAN ANTI-SELF ANTIBODIES WITH HIGH SPECIFICITY FROM PHAGE DISPLAY LIBRARIES
    GRIFFITHS, AD
    MALMQVIST, M
    MARKS, JD
    BYE, JM
    EMBLETON, MJ
    MCCAFFERTY, J
    BAIER, M
    HOLLIGER, KP
    GORICK, BD
    HUGHESJONES, NC
    HOOGENBOOM, HR
    WINTER, G
    [J]. EMBO JOURNAL, 1993, 12 (02) : 725 - 734
12345