Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells Into Germ-Like Cells In Vitro

被引：144
作者：
Huang, Peng [1 ]
Lin, Li Min [1 ]
Wu, Xiao Ying [1 ]
Tang, Qiu Ling [1 ]
Feng, Xue Yong [1 ]
Lin, Guang Yu [1 ]
Lin, Xiaobo [1 ]
Wang, Hong Wu [1 ]
Huang, Tian Hua [2 ]
Ma, Lian [1 ]
机构：
[1] Shantou Univ, Coll Med, Affiliated Hosp 2, Dept Pediat, Shantou 515041, Guangdong, Peoples R China
[2] Shantou Univ, Coll Med, Res Ctr Reprod Med, Shantou 515041, Guangdong, Peoples R China
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2010年 / 109卷 / 04期
基金：
中国国家自然科学基金;
关键词：
HUMAN UMBILICAL CORD; MESENCHYMAL STEM CELL; GERM LIKE CELLS; IN VITRO DIFFERENTIATION; ACID RECEPTOR-ALPHA; BONE-MARROW; RETINOIC ACID; DEVELOPMENTAL EXPRESSION; TRANSCRIPTION FACTOR; CONDITIONED MEDIUM; POTENTIAL ROLE; RAT TESTIS; MICE; DERIVATION;
D O I：
10.1002/jcb.22453
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent studies have demonstrated that mesenchymal stem cells could differentiate into germ cells under appropriate conditions. We sought to determine whether human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) could form germ cells in vitro. HUMSCs were induced to differentiate into germ cells in all-trans retinoic acid, testosterone and testicular-cell-conditioned medium prepared from newborn male mouse testes. HUMSCs formed "tadpole-like" cells after induction with different reagents and showed both mRNA and protein expression of germ-cell-specific markers Oct4 (POUE5), Ckit, CD49(f) (alpha 6), Stella (DDPA3), and Vasa (DDX4). Our results may provide a new route for reproductive therapy involving HUMSCs and a novel in vitro model to investigate the molecular mechanisms that regulate the development of the mammalian germ lineage. J. Cell. Biochem. 109: 747-754, 2010. (C) 2010 Wiley-Liss. Inc.
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收藏
页码：747 / 754
页数：8
相关论文
共 51 条
[1]   Retinoic acid receptor alpha gene expression in the rat testis: Potential role during the prophase of meiosis and in the transition from round to elongating spermatids [J].
Akmal, KM ;
Dufour, JM ;
Kim, KH .
BIOLOGY OF REPRODUCTION, 1997, 56 (02) :549-556
[2]   Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow [J].
Baksh, Dolores ;
Yao, Raphael ;
Tuan, Rocky S. .
STEM CELLS, 2007, 25 (06) :1384-1392
[3]   The human VASA gene is specifically expressed in the germ cell lineage [J].
Castrillon, DH ;
Quade, BJ ;
Wang, TY ;
Quigley, C ;
Crum, CP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (17) :9585-9590
[4]   Modelling germ cell development in vitro [J].
Childs, Andrew J. ;
Saunders, Philippa T. K. ;
Anderson, Richard A. .
MOLECULAR HUMAN REPRODUCTION, 2008, 14 (09) :501-511
[5]   Immunogenicity of umbilical cord tissue-derived cells [J].
Cho, Patricia S. ;
Messina, Darin J. ;
Hirsh, Erica L. ;
Chi, Nina ;
Goldman, Stephanie N. ;
Lo, Diana P. ;
Harris, Ian R. ;
Popma, Sicco H. ;
Sachs, David H. ;
Huang, Christene A. .
BLOOD, 2008, 111 (01) :430-438
[6]   Spontaneous differentiation of germ cells from human embryonic stem cells in vitro [J].
Clark, AT ;
Bodnar, MS ;
Fox, M ;
Rodriquez, RT ;
Abeyta, MJ ;
Firpo, MT ;
Pera, RAR .
HUMAN MOLECULAR GENETICS, 2004, 13 (07) :727-739
[7]  
Conti M, 2008, NAT MED, V14, P1188, DOI 10.1038/nm1108-1188
[8]   Maintenance of adult mouse type A spermatogonia in vitro:: influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture [J].
Creemers, LB ;
den Ouden, K ;
van Pelt, AMM ;
de Rooij, DG .
REPRODUCTION, 2002, 124 (06) :791-799
[9]   Derivation of oocyte-like cells from a clonal pancreatic stem cell line [J].
Danner, S. ;
Kajahn, J. ;
Geismann, C. ;
Klink, E. ;
Kruse, C. .
MOLECULAR HUMAN REPRODUCTION, 2007, 13 (01) :11-20
[10]   Embryonic stem cells: An exciting field for basic research and tissue engineering, but also an ethical dilemma? [J].
Denker, HW .
CELLS TISSUES ORGANS, 1999, 165 (3-4) :246-249
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