DREADDs for Neuroscientists

被引：1143

作者：

Roth, Bryan L. ^{[1
]}

机构：

[1] Univ North Carolina Chapel Hill, Sch Med, Dept Pharmacol, Chapel Hill, NC 27514 USA

来源：

NEURON | 2016年 / 89卷 / 04期

关键词：

BETA(2) ADRENERGIC-RECEPTOR; ATYPICAL ANTIPSYCHOTIC AGENTS; CHEMICAL-GENETIC APPROACH; CLOZAPINE-N-OXIDE; BETA(2)-ADRENERGIC RECEPTOR; REMOTE-CONTROL; CONDITIONAL EXPRESSION; G(Q)-COUPLED RECEPTOR; G(I)-COUPLED RECEPTOR; STRUCTURAL INSIGHTS;

D O I：

10.1016/j.neuron.2016.01.040

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To understand brain function, it is essential that we discover how cellular signaling specifies normal and pathological brain function. In this regard, chemogenetic technologies represent valuable platforms for manipulating neuronal and non-neuronal signal transduction in a cell-type-specific fashion in freely moving animals. Designer Receptors Exclusively Activated by Designer Drugs (DREADD)-based chemogenetic tools are now commonly used by neuroscientists to identify the circuitry and cellular signals that specify behavior, perceptions, emotions, innate drives, and motor functions in species ranging from flies to nonhuman primates. Here I provide a primer on DREADDs highlighting key technical and conceptual considerations and identify challenges for chemogenetics going forward.

引用

页码：683 / 694

页数：12

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