Phenotypic characterization of lactic acid bacteria from sourdoughs for Altamura bread produced in Apulia (Southern Italy)

被引:48
作者
Ricciardi, A [1 ]
Parente, E [1 ]
Piraino, P [1 ]
Paraggio, M [1 ]
Romano, P [1 ]
机构
[1] Univ Basilicata, Dipartimento Chim Difesa & Biotecnol Agroforestal, Campus Macchia Romana, I-85100 Potenza, Italy
关键词
D O I
10.1016/j.ijfoodmicro.2004.05.007
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
In order to study the composition of the lactic acid bacteria (LAB) community of sourdoughs used for the manufacture of Altamura bread, a traditional durum wheat bread produced in Apulia (Southern Italy), 111 strains of LAB were isolated and characterized. The phenotypic characterization of the isolates, carried out using a set of 29 tests, allowed the identification of 15 clusters at the 80% similarity level by hierarchical cluster analysis. Of the isolates, 88% were identified as facultatively heterofermentative LAB (Lactobacillus plantarum, Lb. paracasei, Lb. casei) and 12% as heterofermentative LAB (Lb. brevis, Leuconostoc mesenteroides). SDS-PAGE profiles of whole cell proteins of 68 strains confirmed the identification. Both the diversity and structure of the lactic microflora for sourdoughs for Altamura bread varied among samples. © 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:63 / 72
页数:10
相关论文
共 29 条
[1]  
Coppola S., 1996, Advances in Food Sciences, V18, P160
[2]   Antibacterial activity of sourdough lactic acid bacteria: Isolation of a bacterioincin-like inhibitory substance from Lactobacillus sanfrancisco C57 [J].
Corsetti, A ;
Gobbetti, M ;
Smacchi, E .
FOOD MICROBIOLOGY, 1996, 13 (06) :447-456
[3]   Phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species Triticum durum and Triticum aestivum) sourdoughs of Southern Italy [J].
Corsetti, A ;
Lavermicocca, P ;
Morea, M ;
Baruzzi, F ;
Tosti, N ;
Gobbetti, M .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 64 (1-2) :95-104
[4]   Characterization of reutericyclin produced by Lactobacillus reuteri LTH2584 [J].
Gänzle, MG ;
Höltzel, A ;
Walter, J ;
Jung, G ;
Hammes, WP .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4325-4333
[5]   The sourdough microflora: Interactions of lactic acid bacteria and yeasts [J].
Gobbetti, M .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1998, 9 (07) :267-274
[6]  
GOBBETTI M, 1995, ITAL J FOOD SCI, V7, P91
[7]  
GOBBETTI M, 1995, ITAL J FOOD SCI, V7, P103
[8]  
Gobbetti M., 1994, Ital. J. Food Sci., V6, P85
[9]  
Hammes W. P., 1992, PROCARYOTES HDB BIOL, V2, P1508
[10]  
HARRIGAN W.F., 1976, LAB METHODS FOOD DAI, P320