A Highly Sensitive Hydrogen Peroxide Biosensor Based on Hemoglobin Immobilized on Cadmium Sulfide Quantum Dots/Chitosan Composite Modified Glassy Carbon Electrode

被引：13

作者：

Butwong, Nutthaya ^{[1
]}

Zhou, Lin ^{[2
,3
]}

Moore, Eric ^{[2
,3
,4
,5
]}

Srijaranai, Supalax ^{[4
,5
]}

Luong, John H. T. ^{[2
,3
]}

Glennon, Jeremy D. ^{[2
,3
]}

机构：

[1] Rajamangala Univ Technol, Fac Sci & Liberal Arts, Dept Appl Chem, Nakhon 30000, Ratchasima, Thailand

[2] Natl Univ Ireland Univ Coll Cork, Innovat Chromatog Grp, ISSC, Dept Chem, Cork, Ireland

[3] Natl Univ Ireland Univ Coll Cork, ABCRF, Cork, Ireland

[4] Khon Kaen Univ, Fac Sci, Dept Chem, Khon Kaen 40002, Thailand

[5] Khon Kaen Univ, Ctr Innovat Chem, Fac Sci, Khon Kaen 40002, Thailand

来源：

ELECTROANALYSIS | 2014年 / 26卷 / 11期

基金：

爱尔兰科学基金会;

关键词：

Hemoglobin; Cadmium sulphide; Quantum dots; Chitosan; Hydrogen peroxide; LAYER-BY-LAYER; DIRECT ELECTROCHEMISTRY; FLUORESCENCE PROBE; AQUEOUS-SOLUTION; IONIC LIQUID; CHITOSAN; DOTS; NANOPARTICLES; NANOTUBES; FILMS;

D O I：

10.1002/elan.201400353

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An L-cysteine capped cadmium sulfide-chitosan nanocomposite has been synthesized, characterized and used for surface modification of a glassy carbon electrode. With direct electron transfer, hemoglobin (Hb) adsorbed strongly on the nanocomposite and displayed excellent bioelectrocatalysis for H2O2. The biosensor was capable of reducing H2O2 at -0.35 V, with a detection limit of 3.13 nM, linearity in the range of 15 nM to 10 mu M and a response time of less than 2 s. The Michaelis-Menten constant (K-M) was 0.57 nM, attesting high bioelectrocatalysis of immobilized Hb for H2O2. Reproducibility of the fabrication method was very satisfactory with a relative standard deviation of 5.3%. The biosensor lost only 6.5% of its original response after 7 days when stored in a pH 7.4 PBS at 4 degrees C.

引用

页码：2465 / 2473

页数：9

共 34 条

[1] Preparation, characterization and evaluation of water-soluble L-cysteine-capped-CdS nanoparticles as fluorescence probe for detection of Hg(II) in aqueous solution [J].