Rapid and direct sequencing of double-stranded DNA using exonuclease III and MALDI-TOF MS

被引：21
|
作者：
Puapaiboon, U
Jai-nhuknan, J
Cowan, JA
机构：
[1] Ohio State Univ, Evans Lab Chem, Columbus, OH 43210 USA
[2] Bruker Dalton Inc, Fremont, CA USA
来源：
ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 14期
关键词：
D O I：
10.1021/ac000181n
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Application of MALDI-TOF MS to direct sequencing of dsDNA substrates is demonstrated using a strategy that employs exonuclease III digestion of a target sequence. Experimental conditions for exonuclease III have been optimized for this application, including addition of essential divalent metal ion cofactors. A short cation-exchange column was designed to provide efficient sample cleanup and overcome major problems arising from salt interference.
引用
收藏
页码：3338 / 3341
页数：4
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