Overexpression of a peanut NAC gene, AhNAC4, confers enhanced drought tolerance in tobacco

被引:29
作者
Tang, G. Y. [1 ]
Shao, F. X. [2 ]
Xu, P. L. [1 ]
Shan, L. [1 ]
Liu, Z. J. [3 ]
机构
[1] Shandong Acad Agr Sci, Biotech Res Ctr, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Peoples R China
[2] Shandong Normal Univ, Sch Life Sci, Jinan 250358, Peoples R China
[3] Shandong Acad Agr Sci, Shandong Cotton Res Ctr, Jinan 250100, Peoples R China
基金
中国国家自然科学基金;
关键词
Arachis hypogaea; NAC transcription factor; transgenic tobacco; drought tolerance; TRANSCRIPTION FACTOR; MOLECULAR CHARACTERIZATION; FUNCTIONAL-ANALYSIS; GLYCINE-MAX; STRESS; EXPRESSION; PROTEIN; FAMILY; RESPONSES; DISTINCT;
D O I
10.1134/S1021443717040161
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
NAC is one of the most abundant plant-specific groups of transcription factors, which play important roles in plant growth and development regulation, as well as in biotic and abiotic stress responses. In the present study, a stress-responsive NAC gene, AhNAC4, was isolated from a cDNA library of peanut (Arachis hypogaea L.) immature seeds, and characterized for its role in drought tolerance. AhNAC4 shared high amino acid similarity with NAC proteins belonging to the ATAF subfamily. The expression analysis indicated that AhNAC4 was highly induced by drought, salinity and ABA treatments. Transient expression analysis showed the AhNAC4-GFP fusion protein was exclusively localized in the nucleus of onion epidermal cells. Transactivation assays in yeast cells demonstrated that AhNAC4 functioned as a transcription activator and its C-terminus contained the activation domain. Overexpression of AhNAC4 confers enhanced drought tolerance in transgenic tobacco plants. The improved drought tolerance was associated with more stomatal closure and higher water use efficiency. Collectively, our results indicated that AhNAC4 functions as an important regulator in response to drought stress.
引用
收藏
页码:525 / 535
页数:11
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