Kushneria sinocarnis sp nov., a moderately halophilic bacterium isolated from a Chinese traditional cured meat

A Gram-negative, aerobic, moderately halophilic bacterium, designated strain Z35(T), was isolated from a Chinese traditional cured meat produced in Wuhan. The isolate grew with 1-20% NaCl (optimum 10%), at 4-42 degrees C (optimum 37 degrees C) and at pH 4.5-8.5 (optimum pH 7.0). Cells of strain Z35(T) were not motile and were rod or oval shaped. The genomic DNA G+C content was 59.1 mol%. The isoprenoid quinones were Q-9 (88.96%), 0-8 (9.46 %) and Q-10 (1.58%). The major fatty acids were C-19:0 cyclo omega 8c, C-16:0 and C-17:0 cyclo. Phylogenetic analysis based on 16S rRNA gene sequence analysis indicated that strain Z35(T) was closely related to the type strains of Kushneria species with 93.7-95.3% sequence similarities. Phylogenetic analysis based on 23S rRNA gene sequence similarity values also confirmed the phylogenetic position of the isolate. Therefore, based on the phenotypic, chemotaxonomic and phylogenetic evidence, strain Z35(T) is affiliated to Kushneria, but is clearly differentiated from other species of this genus and represents a new member, for which the name Kushneria sinocarnis sp. nov. is proposed. The type strain is Z35(T) (=CCTCC AB 209027(T) =NRRL B-59197(T) =DSM 23229(T)).