Multifunctional antioxidant activity of polysaccharide fractions from the soybean byproduct okara

被引:177
作者
Mateos-Aparicio, I. [1 ]
Mateos-Peinado, C. [1 ]
Jimenez-Escrig, A. [1 ]
Ruperez, P. [1 ]
机构
[1] CSIC, Metab & Nutr Dept, Inst Frio IF ICTAN, E-28040 Madrid, Spain
关键词
Soybean; Glycine max; Okara; Byproduct; Cell-wall polysaccharides; Antioxidant activity; CELL-WALL POLYSACCHARIDES; DIETARY FIBER; EXTRACTS; CAPACITY; HEALTH; WATER; ACIDS; MEAL; L;
D O I
10.1016/j.carbpol.2010.04.020
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
An alcohol insoluble residue (AIR) from okara, a byproduct from soymilk, was mainly composed by indigestible carbohydrate (55.7%). After sequential extraction, three alkali-soluble fractions (12.7% yield) and an insoluble residue (RES, 58.7% yield) were obtained. Soluble polysaccharide fractions showed in vitro reduction power (11-26 mu mol Trolox Equivalent (TE)/g dry weight (dw)) and free radical scavenging activity (63-78 mu mol TE/g dw). The highest antioxidant activity was exhibited by 0.05 M NaOH-soluble fraction, rich in pectins. The 1 M KOH-soluble fraction had a mixture of hemicellulosic and pectic polysaccharides, and 4M KOH-soluble fraction contained the bulk of xyloglucans, although some pectins could also be present. RES contained cellulose along with residual pectins. FT-IR spectra of okara and AIR exhibited an absorption band at 1740 cm(-1) of carboxylic ester from pectins, which lacked in the fractions. Moreover, they showed absorption bands at 1650 and 1550 cm(-1) of proteins, and at 900-890 cm(-1) of beta-glycosidic linkages. Potential antioxidant activity of okara cell-wall polysaccharides could be attributed to pectins, although the contribution of residual proteins cannot be ruled out. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:245 / 250
页数:6
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