Carbon nanotubes as scaffolds for cell culture and effect on cellular functions

被引：69

作者：

Aoki, Naofumi ^{[1
]}

Akasaka, Tsukasa ^{[1
]}

Watari, Fumio ^{[1
]}

Yokoyama, Atsuro ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Dent Med, Kita Ku, Sapporo, Hokkaido 0608586, Japan

来源：

DENTAL MATERIALS JOURNAL | 2007年 / 26卷 / 02期

关键词：

carbon nanotubes; scaffold; osteoblast;

D O I：

10.4012/dmj.26.178

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

To investigate the dependence of blocompatibility of carbon materials on crystal structure with the aim of developing biomedical applications, single-(SW) and multi-walled (MW) carbon nanotubes (CNTs) were employed as scaffolds for cell culture and compared with graphite (GP). SaOS2 cells were used to investigate the properties and response of osteoblast-like cells. Polycarbonate membranes (PC) coated with CNTs by vacuum filtration formed a meshwork nanostructure. Cells grown on CNTs greatly extended in all directions. In terms of cell proliferation, alkaline phosphatase (ALP) activity, and protein adsorption on the substrates, CNTs showed better results than PC and GP. SW showed the best cell proliferation and total ALP. These favorable results might be attributed to the structure of CNTs and the affinity of CNTs toward proteins, thereby suggesting that CNTs could be potential scaffold materials for cell culture.

引用

页码：178 / 185

页数：8

共 37 条

[1] Nano-architecture on carbon nanotube surface by biomimetic coating [J].

Akasaka, T ;

Watari, F .

CHEMISTRY LETTERS, 2005, 34 (06) :826-827

[2] Osteoblast adhesion on biomaterials [J].

Anselme, K .

BIOMATERIALS, 2000, 21 (07) :667-681

[3] Strikingly extended morphology of cells grown on carbon nanotubes [J].

Aoki, Naofumi ;

Yokoyama, Atsuro ;

Nodasaka, Yoshinobu ;

Akasaka, Tsukasa ;

Uo, Motohiro ;

Sato, Yoshinori ;

Tohji, Kazuyuki ;

Watari, Fumio .

CHEMISTRY LETTERS, 2006, 35 (05) :508-509

[4] Cell Culture on a Carbon Nanotube Scaffold [J].

Aoki, Naofurni ;

Yokoyama, Atsuro ;

Nodasaka, Yoshinobu ;

Akasaka, Tsukasa ;

Uo, Motohiro ;

Sato, Yoshinori ;

Tohji, Kazuyuki ;

Watari, Furnio .

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2005, 1 (04) :402-405

[5] Applications of carbon nanotubes in drug delivery [J].

Bianco, A ;

Kostarelos, K ;

Prato, M .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2005, 9 (06) :674-679

[6] Multi-walled carbon nanotubes induce T lymphocyte apoptosis [J].

Bottini, M ;

Bruckner, S ;

Nika, K ;

Bottini, N ;

Bellucci, S ;

Magrini, A ;

Bergamaschi, A ;

Mustelin, T .

TOXICOLOGY LETTERS, 2006, 160 (02) :121-126

[7] In vitro studies of carbon nanotubes biocompatibility [J].

Chlopek, J ;

Czajkowska, B ;

Szaraniec, B ;

Frackowiak, E ;

Szostak, K ;

Béguin, F .

CARBON, 2006, 44 (06) :1106-1111

[8] Fabrication and biocompatibility of carbon nanotube-based 3D networks as scaffolds for cell seeding and growth [J].

Correa-Duarte, MA ;

Wagner, N ;

Rojas-Chapana, J ;

Morsczeck, C ;

Thie, M ;

Giersig, M .

NANO LETTERS, 2004, 4 (11) :2233-2236

[9] Effect of single wall carbon nanotubes on human HEK293 cells [J].

Cui, DX ;

Tian, FR ;

Ozkan, CS ;

Wang, M ;

Gao, HJ .

TOXICOLOGY LETTERS, 2005, 155 (01) :73-85

[10] Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength [J].

Deligianni, DD ;

Katsala, ND ;

Koutsoukos, PG ;

Missirlis, YF .

BIOMATERIALS, 2001, 22 (01) :87-96

← 1 2 3 4 →