Multiphoton Microscopy of Live Tissues With Ultraviolet Autofluorescence

被引：25

作者：

Li, Chunqiang ^{[1
,2
]}

Pitsillides, Costas ^{[3
,4
]}

Runnels, Judith M. ^{[3
,5
]}

Cote, Daniel ^{[6
]}

Lin, Charles P. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Wellman Ctr Photomed,Adv Microscopy Program, Boston, MA 02114 USA

[2] Massachusetts Gen Hosp, Ctr Syst Biol, Boston, MA 02114 USA

[3] Harvard Univ, Sch Med, Wellman Ctr Photomed, Adv Microscopy Program, Boston, MA 02114 USA

[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[5] Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA

[6] Univ Laval, Ctr Rech Univ Laval Robert Giffard, Dept Phys, Quebec City, PQ G1J 2G3, Canada

来源：

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS | 2010年 / 16卷 / 03期

基金：

美国国家卫生研究院;

关键词：

In vivo imaging; multiphoton-excited fluorescence microscopy (MPM); tryptophan autofluorescence; video-rate imaging; EXCITATION FLUORESCENCE MICROSCOPY; IN-VIVO; 2-PHOTON EXCITATION; IMMUNE-SYSTEM; HUMAN SKIN; SEROTONIN; CELLS; TRYPTOPHAN; MELANIN; NADH;

D O I：

10.1109/JSTQE.2009.2031619

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Current research on multiphoton autofluorescence microscopy is primarily focused on imaging the signal from reduced nicotinamide adenine dinucleatide (NADH) in tissue. NADH levels in cells are useful reporters of metabolic information, as well as early indicators in precancer and cancer diagnosis. While NADH is typically imaged in the 400-500 nm spectral window, the amino acid tryptophan is the major source of tissue fluorescence in the Ultraviolet range. Here, we briefly review current progress in multiphoton autofluorescence imaging of live tissues and cells, and report our recent findings of in vivo mouse skin imaging based on multiphoton excited tryptophan autofluorescence. This new method enables noninvasive imaging of skin tissue at video-rate and allows for the visualization and identification of cellular components in the epidermis, dermis, and muscle layers. It is also possible to image through small blood vessels in the mouse skin and observe circulating leukocytes in situ.

引用

页码：516 / 523

页数：8

共 64 条

[1] Quantitative measurement of serotonin synthesis and sequestration in individual live neuronal cells [J].