Dielectrophoretic-activated cell sorter based on curved microelectrodes

被引:53
作者
Khoshmanesh, Khashayar [1 ]
Zhang, Chen [2 ]
Tovar-Lopez, Francisco J. [2 ]
Nahavandi, Saeid [1 ]
Baratchi, Sara [3 ]
Mitchell, Arnan [2 ]
Kalantar-Zadeh, Kourosh [2 ]
机构
[1] Deakin Univ, Ctr Intelligent Syst Res, Geelong, Vic 3217, Australia
[2] RMIT Univ, Sch Elect & Comp Engn, Melbourne, Vic 3001, Australia
[3] Deakin Univ, Inst Biotechnol BioDeakin, Geelong, Vic 3217, Australia
关键词
Dielectrophoresis; Microfluidics; Yeast; Sorting; ELECTRIC-FIELD; YEAST-CELLS; MICROFLUIDIC DEVICE; SINGLE-CELL; RARE CELLS; SEPARATION; MANIPULATION; PARTICLES; CHIP; FLOW;
D O I
10.1007/s10404-009-0558-7
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
This article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of similar to 80% at low medium conductivities and similar to 85% at high medium conductivities.
引用
收藏
页码:411 / 426
页数:16
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