Aptamer-Mediated Chemiluminescence Detection of Prion Protein on a Membrane Using Trimethoxyphenylglyoxal

被引：11

作者：

Hossain, Md Towhid ^{[1
]}

Shibata, Takayuki ^{[1
]}

Kabashima, Tsutomu ^{[1
]}

Kai, Masaaki ^{[1
,2
]}

机构：

[1] Nagasaki Univ, Grad Sch Biomed Sci, Fac Pharmaceut Sci, Nagasaki 8528521, Japan

[2] Nagasaki Univ, Global Ctr Excellence Program, Nagasaki 8528521, Japan

来源：

ANALYTICAL SCIENCES | 2010年 / 26卷 / 06期

关键词：

REAGENT; PRPSC;

D O I：

10.2116/analsci.26.645

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt-Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot(-1).

引用

页码：645 / 647

页数：3

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