Procedure optimization for extracting short-chain fatty acids from human faeces

被引：19

作者：

Dobrowolska-Iwanek, Justyna ^{[1
]}

Zagrodzki, Pawel ^{[1
,2
]}

Wozniakiewicz, Michal ^{[3
]}

Wozniakiewcz, Aneta ^{[3
]}

Zwolinska-Wcislo, Malgorzata ^{[4
]}

Winnicka, Diana ^{[1
]}

Pasko, Pawel ^{[1
,5
]}

机构：

[1] Jagiellonian Univ, Coll Med, Dept Food Chem & Nutr, Med 9, PL-30688 Krakow, Poland

[2] Polish Acad Scienece, Inst Nucl Phys, Dept Nucl Phys Chem, Radzikowskiego 152, PL-31142 Krakow, Poland

[3] Jagiellonian Univ, Fac Chem, Lab Forens Chem, Ingardena 3, PL-30060 Krakow, Poland

[4] Jagiellonian Univ, Coll Med, Dept Gastroenterol Hepatol & Infect Dis, Sniadeckich 5, PL-31501 Krakow, Poland

[5] Andrzej Frycz Modrzewsld Krakow Univ, Fac Hlth & Med Sci, Herlinga Grudzinskiego 1, PL-30705 Krakow, Poland

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2016年 / 124卷

关键词：

Short-chain fatty acids; Extraction; Optimization; Stool; COLONIC HEALTH; MICROBIOTA;

D O I：

10.1016/j.jpba.2016.02.042

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Short-chain fatty acids play an important role in the physiology and metabolism of the colon. Disturbed balance of such compounds in human gut can significantly contribute to etiological factors of various gastrointestinal disorders and it may also increase the risk of developing cancer or cardiovascular diseases. The aim of the study was to select the optimal parameters for acetic, propionic and butyric acids extraction from stool samples. The experimental conditions were optimized with respect to the solvent sample shacking time, sample ultrasounds (Ultrasound-Assisted Extraction, UAE) exposure time and the number of extractions from the particulate stool samples. The screening of experimental parameters was conducted with fractional factorial design of experiments, namely 3(3-1). The optimal conditions for UAE were found, namely ultrasound digestion time of 40 min (at 35 degrees C), shaking time of 4 min, and the three subsequent extractions. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：337 / 340

页数：4

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