Toxoplasma gondii in tissues of sheep slaughtered for human consumption in a highly endemic area for toxoplasmosis in Brazil

被引:3
作者
Silva, A. F. [1 ]
Frazao-Teixeira, E. [2 ,3 ]
Oliveira, F. C. R. [4 ]
Fonseca, A. B. M. [5 ]
Ferreira, A. M. R. [6 ]
机构
[1] Univ Fed Rural Rio de Janeiro, Dept Med & Cirurgia Vet, Programa Posgrad Med Vet, Rio De Janeiro, RJ, Brazil
[2] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Biol Estrutural, Av Brasil 4365, BR-21040361 Rio De Janeiro, RJ, Brazil
[3] Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol Agr, Lab Sanidade Anim, Av Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ, Brazil
[4] Univ Estadual Norte Fluminense, Lab Sanidade Anim, Campos Dos Goytacazes, RJ, Brazil
[5] Univ Fed Fluminense, Dept Estat, Rio De Janeiro, RJ, Brazil
[6] Univ Fed Fluminense, Lab Anat Patol Vet, Programa Posgrad Med Vet, Rio De Janeiro, RJ, Brazil
关键词
Toxoplasmosis; Sheep tissues; PCR; MAT; Rio de Janeiro; RIO-DE-JANEIRO; ANTIBODIES; CATTLE; PIGS; PREVALENCE; STATE;
D O I
10.1016/j.vprsr.2022.100688
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Toxoplasma gondii infects warm-blooded animals, including humans, and the ingestion of undercooked meat of infected animals is an important source of infection. We investigated the presence of T. gondii by PCR and histopathology in tissues of 25 seropositive sheep slaughtered for human consumption in Rio de Janeiro and assessed the suitability of different tissues for molecular detection of the parasite. The animals were first screened for T. gondii antibodies by MAT (Modified Agglutination Test), and tissues of seropositive sheep (brain, heart, lungs, kidneys, liver and diaphragm) were subjected to molecular and histological examination. A nested-PCR targeting the P43 gene of T. gondii was performed, and kappa Coefficient was used to assess PCR results among tissues. DNA of the parasite was detected in 60 % (9 of 15) of the animals. and tissue pairs of lungs/heart, lungs/diaphragm or heart/diaphragm had substantial agreement with the global status of the animals. The combination between these three tissues leads to an almost perfect agreement with global status results. Sheep slaughtered for human consumption in Rio de Janeiro, Brazil are infected with T. gondii, and pairs of fragments of lungs and heart, lungs and diaphragm or heart and diaphragm could be used for molecular identification of T. gondii in sheep with substantial agreement with the global status of the animals.
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