Expression and partial biochemical characterization of a recombinant serine protease from Bothrops pauloensis snake venom

被引：6

作者：

Isabel, Thais F. ^{[1
]}

Moreira Costa, Guilherme Nunes ^{[1
]}

Pacheco, Isabela B. ^{[1
]}

Barbosa, Luana G. ^{[1
]}

Santos-Junior, Celio D. ^{[1
]}

Fonseca, Fernando P. P. ^{[2
]}

Franca, Johara Boldrini ^{[3
]}

Henrique-Silva, Flavio ^{[2
]}

Yoneyama, Kelly A. G. ^{[1
]}

Rodrigues, Renata S. ^{[1
,4
]}

Rodrigues, Veridiana de Melo ^{[1
,4
]}

机构：

[1] Univ Fed Uberlandia, Inst Genet & Bioquim, Av Engenheiro Dinz 1178,CP 593, BR-38400 Uberlandia, MG, Brazil

[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13560 Sao Carlos, SP, Brazil

[3] Univ Sao Paulo, Dept Fis & Quim, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP, Brazil

[4] INCT, Inst Nacl Ciencia & Tecnol Nanobiofarmaceut, Belo Horizonte, MG, Brazil

来源：

TOXICON | 2016年 / 115卷

关键词：

Bothrops pauloensis; Snake venom; Thrombin-like; serine protease; Heterologous expression; THROMBIN-LIKE ENZYME; FUNCTIONAL-CHARACTERIZATION; PURIFICATION; CLONING; BATROXOBIN; PROTEINS; CDNA; INSULARIS; COMPLEX; TOXINS;

D O I：

10.1016/j.toxicon.2016.03.002

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Snake venom serine proteases (SVSPs) are enzymes capable of interfering at several points of hemostasis. Some serine proteases present thrombin-like activity, which makes them targets for the development of therapeutics agents in the treatment of many hemostatic disorders. In this study, a recombinant thrombin-like serine protease, denominated rBpSP-II, was obtained from cDNA of the Bothrops pauloensis venom gland and was characterized enzymatically and biochemically. The enzyme rBpSP-II showed clotting activity on bovine plasma and proteolytic activity on fibrinogen, cleaving exclusively the A alpha chain. The evaluation of rBpSP-II activity on chromogenic substrates demonstrated thrombin-like activity of the enzyme due to its capacity to hydrolyze the thrombin substrate. These characteristics make rBpSP-II an attractive molecule for additional studies. Further research is needed to verify whether rBpSP-II can serve as a template for the synthesis of therapeutic agents to treat hemostatic disorders. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：49 / 54

页数：6

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