Expression and partial biochemical characterization of a recombinant serine protease from Bothrops pauloensis snake venom

被引：6
|
作者：
Isabel, Thais F. [1 ]
Moreira Costa, Guilherme Nunes [1 ]
Pacheco, Isabela B. [1 ]
Barbosa, Luana G. [1 ]
Santos-Junior, Celio D. [1 ]
Fonseca, Fernando P. P. [2 ]
Franca, Johara Boldrini [3 ]
Henrique-Silva, Flavio [2 ]
Yoneyama, Kelly A. G. [1 ]
Rodrigues, Renata S. [1 ,4 ]
Rodrigues, Veridiana de Melo [1 ,4 ]
机构：
[1] Univ Fed Uberlandia, Inst Genet & Bioquim, Av Engenheiro Dinz 1178,CP 593, BR-38400 Uberlandia, MG, Brazil
[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13560 Sao Carlos, SP, Brazil
[3] Univ Sao Paulo, Dept Fis & Quim, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP, Brazil
[4] INCT, Inst Nacl Ciencia & Tecnol Nanobiofarmaceut, Belo Horizonte, MG, Brazil
来源：
TOXICON | 2016年 / 115卷
关键词：
Bothrops pauloensis; Snake venom; Thrombin-like; serine protease; Heterologous expression; THROMBIN-LIKE ENZYME; FUNCTIONAL-CHARACTERIZATION; PURIFICATION; CLONING; BATROXOBIN; PROTEINS; CDNA; INSULARIS; COMPLEX; TOXINS;
D O I：
10.1016/j.toxicon.2016.03.002
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Snake venom serine proteases (SVSPs) are enzymes capable of interfering at several points of hemostasis. Some serine proteases present thrombin-like activity, which makes them targets for the development of therapeutics agents in the treatment of many hemostatic disorders. In this study, a recombinant thrombin-like serine protease, denominated rBpSP-II, was obtained from cDNA of the Bothrops pauloensis venom gland and was characterized enzymatically and biochemically. The enzyme rBpSP-II showed clotting activity on bovine plasma and proteolytic activity on fibrinogen, cleaving exclusively the A alpha chain. The evaluation of rBpSP-II activity on chromogenic substrates demonstrated thrombin-like activity of the enzyme due to its capacity to hydrolyze the thrombin substrate. These characteristics make rBpSP-II an attractive molecule for additional studies. Further research is needed to verify whether rBpSP-II can serve as a template for the synthesis of therapeutic agents to treat hemostatic disorders. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：49 / 54
页数：6
相关论文
共 50 条
  • [1] Biochemical and functional characterization of BmooSP, a new serine protease from Bothrops moojeni snake venom
    de Oliveira, Fabio
    de Sousa, Bruna Barbosa
    Neves Mamede, Carla Cristine
    Comes de Morais, Nadia Cristina
    de Queiroz, Mayara Ribeiro
    da Cunha Pereira, Deborah F.
    Matias, Mariana S.
    Homi Brandeburgo, Maria Ines
    TOXICON, 2016, 111 : 130 - 138
  • [2] Biochemical and functional characterization of Bothropoidin: the first haemorrhagic metalloproteinase from Bothrops pauloensis snake venom
    Gomes, Mario Sergio R.
    Naves de Souza, Dayane L.
    Guimaraes, Denise O.
    Lopes, Daiana S.
    Mamede, Carla C. N.
    Gimenes, Sarah Natalie C.
    Ache, David C.
    Rodrigues, Renata S.
    Yoneyama, Kelly A. G.
    Borges, Marcia H.
    de Oliveira, Fabio
    Rodrigues, Veridiana M.
    JOURNAL OF BIOCHEMISTRY, 2015, 157 (03): : 137 - 149
  • [3] Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom
    Zaqueo, Kayena D.
    Kayano, Anderson M.
    Simoes-Silva, Rodrigo
    Moreira-Dill, Leandro S.
    Fernandes, Carla F. C.
    Fuly, Andre L.
    Maltarollo, Vinicius G.
    Honorio, Kathia M.
    da Silva, Saulo L.
    Acosta, Gerardo
    Caballol, Maria Antonia O.
    de Oliveira, Eliandre
    Albericio, Fernando
    Calderon, Leonardo A.
    Soares, Andreimar M.
    Stabeli, Rodrigo G.
    BIOMED RESEARCH INTERNATIONAL, 2014, 2014
  • [4] Biochemical and functional characterization of a C-type lectin (BpLec) from Bothrops pauloensis snake venom
    Castanheira, Leticia Eulalio
    de Oliveira Nunes, Debora Cristina
    Cardoso, Thomaz Monteiro
    Santos, Paula de Souza
    Goulart, Luiz Ricardo
    Rodrigues, Renata Santos
    Richardson, Michael
    Borges, Marcia Helena
    Geraldo Yoneyama, Kelly Aparecida
    Rodrigues, Veridiana M.
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2013, 54 : 57 - 64
  • [5] Hesperetin as an inhibitor of the snake venom serine protease from Bothrops jararaca
    dos Santos, Roney Vander
    Grillo, Giovanna
    Fonseca, Henrique
    Stanisic, Danijela
    Tasic, Ljubica
    TOXICON, 2021, 198 : 64 - 72
  • [6] Biochemical and functional properties of a thrombin-like enzyme isolated from Bothrops pauloensis snake venom
    Costa, Fabio L. S.
    Rodrigues, Renata S.
    Izidoro, Luiz F. M.
    Menaldo, Danilo L.
    Hamaguchi, Amelia
    Homsi-Brandeburgo, Maria I.
    Fuly, Andre L.
    Soares, Sandro G.
    Selistre-de-Araujo, Heloisa S.
    Barraviera, Benedito
    Soares, Andreimar M.
    Rodrigues, Veridiana M.
    TOXICON, 2009, 54 (06) : 725 - 735
  • [7] ISOLATION AND PARTIAL BIOCHEMICAL AND PHARMACOLOGICAL CHARACTERIZATION OF A MYOTOXIN FROM THE VENOM OF THE SNAKE BOTHROPS-NUMMIFER
    GUTIERREZ, JM
    LOMONTE, B
    CERDAS, L
    TOXICON, 1987, 25 (02) : 143 - 143
  • [8] Biochemical characterization and comparative analysis of two distinct serine proteases from Bothrops pirajai snake venom
    Menaldo, Danilo Luccas
    Bernardes, Carolina Petri
    Santos-Filho, Norival Alves
    Moura, Laura de Andrade
    Fuly, Andre Lopes
    Arantes, Eliane Candiani
    Sampaio, Suely Vilela
    BIOCHIMIE, 2012, 94 (12) : 2545 - 2558
  • [9] Generation and In-planta expression of a recombinant single chain antibody with broad neutralization activity on Bothrops pauloensis snake venom
    Souza, Jessica B.
    Cardoso, Rone
    Almeida-Souza, Hebreia O.
    Carvalho, Camila P.
    Veloso Correia, Lucas Ian
    Faria, Paula Cristina B.
    Araujo, Galber R.
    Mendes, Mirian M.
    Rodrigues, Renata Santos
    Rodrigues, Veridiana M.
    Dandekar, Abhaya M.
    Goulart, Luiz Ricardo
    Nascimento, Rafael
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 149 : 1241 - 1251
  • [10] Analyzing the influence of age and sex in Bothrops pauloensis snake venom
    Tasima, Lidia Jorge
    Hatakeyama, Daniela Miki
    Aguiar, Weslei da Silva
    de Lima, Eduardo Oliveira Venacioio de
    Miyamoto, Jackson Gabriel
    Tashima, Alexandre Keiji
    Grego, Kathleen Fernandes
    de Morais-Zani, Karen
    Tanaka-Azevedo, Anita Mitico
    TOXICON, 2022, 214 : 78 - 90
12345