Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins

被引:54
作者
Yamamoto, Ryosuke [1 ]
Hirono, Masafumi [1 ]
Kamiya, Ritsu [1 ]
机构
[1] Univ Tokyo, Dept Biol Sci, Grad Sch Sci, Tokyo 1130033, Japan
基金
日本学术振兴会;
关键词
INNER-ARM DYNEIN; INTERMEDIATE CHAIN; CHLAMYDOMONAS; MOTILITY; MUTANTS; SUBUNIT; ACTIN; CELL;
D O I
10.1083/jcb.201002081
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Axonemal dyneins are preassembled in the cytoplasm before being transported into cilia and flagella. Recently, PF13/KTU, a conserved protein containing a PIH (protein interacting with HSP90) domain, was identified as a protein responsible for dynein preassembly in humans and Chlamydomonas reinhardtii. This protein is involved in the preassembly of outer arm dynein and some inner arm dyneins, possibly as a cofactor of molecular chaperones. However, it is not known which factors function in the preassembly of other inner arm dyneins. Here, we analyzed a novel C. reinhardtii mutant, ida10, and found that another conserved PIH family protein, MOT48, is responsible for the formation of another subset of inner arm dyneins. A variety of organisms with motile cilia and flagella typically have three to four PIH proteins, including potential homologues of MOT48 and PF13/KTU, whereas organisms without them have no, or only one, such protein. These findings raise the possibility that multiple PIH proteins are commonly involved in the preassembly of different subsets of axonemal dyneins.
引用
收藏
页码:65 / 71
页数:7
相关论文
共 36 条
[1]   The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery [J].
Boulon, Severine ;
Marmier-Gourrier, Nathalie ;
Pradet-Balade, Berengere ;
Wurth, Laurence ;
Verheggen, Celine ;
Jady, Beata E. ;
Rothe, Benjamin ;
Pescia, Christina ;
Robert, Marie-Cecile ;
Kiss, Tamas ;
Bardoni, Barbara ;
Krol, Alain ;
Branlant, Christiane ;
Allmang, Christine ;
Bertrand, Edouard ;
Charpentier, Bruno .
JOURNAL OF CELL BIOLOGY, 2008, 180 (03) :579-595
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   BENDING PATTERNS OF CHLAMYDOMONAS FLAGELLA .4. MUTANTS WITH DEFECTS IN INNER AND OUTER DYNEIN ARMS INDICATE DIFFERENCES IN DYNEIN ARM FUNCTION [J].
BROKAW, CJ ;
KAMIYA, R .
CELL MOTILITY AND THE CYTOSKELETON, 1987, 8 (01) :68-75
[4]   The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii [J].
Fischer, N ;
Rochaix, JD .
MOLECULAR GENETICS AND GENOMICS, 2001, 265 (05) :888-894
[5]   The role of preassembled cytoplasmic complexes in assembly of flagellar dynein subunits [J].
Fowkes, ME ;
Mitchell, DR .
MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (09) :2337-2347
[6]   Characterization of Saccharomyces cerevisiae Nop17p, a novel Nop58p-interacting pre-rRNA processing [J].
Gonzales, FA ;
Zanchin, NIT ;
Luz, JS ;
Oliveira, CC .
JOURNAL OF MOLECULAR BIOLOGY, 2005, 346 (02) :437-455
[8]   IC138 is a WD-repeat dynein intermediate chain required for light chain assembly and regulation of flagellar bending [J].
Hendrickson, TW ;
Perrone, CA ;
Griffin, P ;
Wuichet, K ;
Mueller, J ;
Yang, PF ;
Porter, ME ;
Sale, WS .
MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (12) :5431-5442
[9]   A Novel Ankyrin-Repeat Protein Interacts with the Regulatory Proteins of Inner Arm Dynein f (I1) of Chlamydomonas reinhardtii [J].
Ikeda, Kazuho ;
Yamamoto, Ryosuke ;
Wirschell, Maureen ;
Yagi, Toshiki ;
Bower, Raqual ;
Porter, Mary E. ;
Sale, Winfield S. ;
Kamiya, Ritsu .
CELL MOTILITY AND THE CYTOSKELETON, 2009, 66 (08) :448-456
[10]  
KAGAMI O, 1992, J CELL SCI, V103, P653