Planar displacement chromatography

被引：4

作者：

Kalász, H ^{[1
]}

机构：

[1] Semmelweis Univ, Dept Pharmacol & Pharmacotherapy, H-1089 Budapest, Hungary

来源：

JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2004年 / 17卷 / 06期

关键词：

displacement chromatography; planar chromatography; spacer; N '-monomethylysine; transmethylation;

D O I：

10.1556/JPC.17.2004.6.13

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Displacement chromatography (DC) has been widely used to separate metabolites with similar chemical characteristics. DC works with highly overloaded sample sizes, which are normal for samples not subjected to clean-up. DC successfully handles samples which contain high concentrations of salts and/or proteins, and results in consecutive steps of displaced compounds rather than Gaussian curves. Planar displacement chromatography (DTLC) is suitable for seeking new, i.e. hitherto unknown, metabolites in excreted body fluids. Transfer of the radiolabeled methyl group from L-deprenyl to N-epsilon-monomethylysine can easily be proven using spacer-displacement planar chromatography.

引用

页码：464 / 467

页数：4

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