Role of Bovine Fetal Platelet-rich Plasma (PRP) on Skin Wound Healing in Mice

Background and Objective: Platelet-rich plasma is the portion of the blood plasma which contains platelet concentration beyond usual levels. It is a new method to stimulate tissue regeneration response as it contains a wide range of growth factors that enhance wound healing and tissue repairing mechanism. This study was aimed to investigate the impact of concurrent application of topical and subcutaneous (S/C) infiltration of PRP at the excised wound boundaries upon epidermal surgical lesions. Materials and Methods: A clean epidermal incision of 5 mm in diameter was made in the back region of each mouse (n = 20). Animals were divided in 2 groups, group 1 (n = 10) were left untreated (control group), while group 2 received bovine fetal. Wounds were topically and subcutaneously infiltrated with PRP for 21 days. Wound healing measurement, percentage of wound contraction and histopathological investigation of the wounds from skin biopsy were evaluated, both in treated and un-treated mice at 3rd day, 7th day, 14th day and 21st day post wounding. Results: There was an obvious progress in wound healing response at 14th day of PRP treatment and complete healing after 21 days of PRP treatment, comparing to the control group. Also, the percentage of epithelization and wound contraction were significantly increased in PRP-treated mice at any period of time, comparing to control group. Histopathological results showed higher granulation tissue formation, new blood vessel formation and collagen synthesis. Conclusion: The PRP could be useful to enhance wound repair and reduces scar tissue formation in surgically-induced excised skin wounds.