Stability of global methylation profiles of whole blood and extracted DNA under different storage durations and conditions

被引：40

作者：

Li, Yingchuan ^{[1
]}

Pan, Xiaoqing ^{[1
,2
]}

Roberts, Michelle L. ^{[1
]}

Liu, Pengyuan ^{[1
,3
,4
]}

Kotchen, Theodore A. ^{[5
]}

Cowley, Allen W., Jr. ^{[1
]}

Mattson, David L. ^{[1
]}

Liu, Yong ^{[1
]}

Liang, Mingyu ^{[1
]}

Kidambi, Srividya ^{[5
]}

机构：

[1] Med Coll Wisconsin, Dept Physiol, Ctr Syst Mol Med, 8701 Watertown Plank Rd, Milwaukee, WI 53226 USA

[2] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Crit Care Med, Shanghai, Peoples R China

[3] Zhejiang Univ, Dept Resp Med, Sir Run Run Shaw Hosp, Hangzhou, Zhejiang, Peoples R China

[4] Zhejiang Univ, Inst Translat Med, Hangzhou, Zhejiang, Peoples R China

[5] Med Coll Wisconsin, Dept Med, Milwaukee, WI 53226 USA

来源：

EPIGENOMICS | 2018年 / 10卷 / 06期

关键词：

methylation profiles; stability; storage conditions; DISEASE; TISSUE; SITES; SALT;

D O I：

10.2217/epi-2018-0025

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: To test whether DNA samples stored for a prolonged period (20 years) under various storage conditions could be used for comparative methylation studies using reduced representation bisulfite sequencing. Patients & methods: Five groups of human blood DNA samples (n = 5-6/group) were compared. The groupings were based on the anticoagulant used and storage temperature and duration. Results: Methylation profiles of defined genomic regions in the DNA or blood samples archived for 20 years were similar across all storage temperatures, including 4 degrees C. The level of intersample similarity in archived samples was not significantly different than that in recently collected samples. Conclusion: Archived samples, including DNA stored at 4 degrees C for 20 years, are suitable for comparative studies of DNA methylation.

引用

页码：797 / 811

页数：15

共 42 条

[41] Liquid chromatography and ultra-performance liquid chromatography-mass spectrometry fingerprinting of human urine - Sample stability under different handling and storage conditions for metabonomics studies
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Theodoridis, Georgios A.
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JOURNAL OF CHROMATOGRAPHY A, 2008, 1189 (1-2) : 314 - 322
[42] Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry
Xue, Meng
Shi, Hang
Zhang, Jiao
Liu, Qing-Quan
Guan, Jun
Zhang, Jia-Yu
Ma, Qun
MOLECULES, 2016, 21 (07):

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