Stability of global methylation profiles of whole blood and extracted DNA under different storage durations and conditions

被引：40
作者：
Li, Yingchuan [1 ]
Pan, Xiaoqing [1 ,2 ]
Roberts, Michelle L. [1 ]
Liu, Pengyuan [1 ,3 ,4 ]
Kotchen, Theodore A. [5 ]
Cowley, Allen W., Jr. [1 ]
Mattson, David L. [1 ]
Liu, Yong [1 ]
Liang, Mingyu [1 ]
Kidambi, Srividya [5 ]
机构：
[1] Med Coll Wisconsin, Dept Physiol, Ctr Syst Mol Med, 8701 Watertown Plank Rd, Milwaukee, WI 53226 USA
[2] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Crit Care Med, Shanghai, Peoples R China
[3] Zhejiang Univ, Dept Resp Med, Sir Run Run Shaw Hosp, Hangzhou, Zhejiang, Peoples R China
[4] Zhejiang Univ, Inst Translat Med, Hangzhou, Zhejiang, Peoples R China
[5] Med Coll Wisconsin, Dept Med, Milwaukee, WI 53226 USA
来源：
EPIGENOMICS | 2018年 / 10卷 / 06期
关键词：
methylation profiles; stability; storage conditions; DISEASE; TISSUE; SITES; SALT;
D O I：
10.2217/epi-2018-0025
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Aim: To test whether DNA samples stored for a prolonged period (20 years) under various storage conditions could be used for comparative methylation studies using reduced representation bisulfite sequencing. Patients & methods: Five groups of human blood DNA samples (n = 5-6/group) were compared. The groupings were based on the anticoagulant used and storage temperature and duration. Results: Methylation profiles of defined genomic regions in the DNA or blood samples archived for 20 years were similar across all storage temperatures, including 4 degrees C. The level of intersample similarity in archived samples was not significantly different than that in recently collected samples. Conclusion: Archived samples, including DNA stored at 4 degrees C for 20 years, are suitable for comparative studies of DNA methylation.
引用
收藏
页码：797 / 811
页数：15
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