Impact of adipose tissue or umbilical cord derived mesenchymal stem cells on the immunogenicity of human cord blood derived endothelial progenitor cells

被引:22
作者
Tan, Kefang [1 ,2 ]
Zheng, Ke [1 ,2 ]
Li, Daiye [1 ,2 ]
Lu, Haiyuan [1 ,3 ]
Wang, Siqi [1 ,2 ]
Sun, Xuan [1 ,2 ]
机构
[1] Cent S Univ, Sch Basic Med Sci, Inst Reprod & Stem Cell Engn, Changsha, Hunan, Peoples R China
[2] Natl Engn & Res Ctr Human Stem Cell, Changsha, Hunan, Peoples R China
[3] Cent S Univ, Xiangya Hosp, Changsha, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
STROMAL CELLS; TRANSPLANTATION;
D O I
10.1371/journal.pone.0178624
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The application of autologous endothelial progenitor cell (EPC) transplantation is a promising approach in therapeutic cardiovascular diseases and ischemic diseases. In this study, we compared the immunogenicity of EPCs, adipose tissue (AD)-derived mesenchymal stem cells (MSCs) and umbilical cord (UC)-derived MSCs by flow cytometry and the mixed lymphocyte reaction. The impact of AD-MSCs and UC-MSCs on the immunogenicity of EPCs was analyzed by the mixed lymphocyte reaction and cytokine secretion in vitro and was further tested by allogenic peripheral blood mononuclear cell (PBMC) induced immunorejection on a cell/matrigel graft in an SCID mouse model. EPCs and AD-MSCs express higher levels of MHC class I than UC-MSCs. All three kinds of cells are negative for MHC class II. UC-MSCs also express lower levels of IFN-y receptor mRNA when compared with EPCs and AD-MSCs. EPCs can stimulate higher rates of proliferation of lymphocytes than AD-MSCs and UC-MSCs. Furthermore, AD-MSCs and UC-MSCs can modulate immune response and inhibit lymphocyte proliferation induced by EPCs, mainly through inhibition of the proliferation of CD8+ T cells. Compared with UC-MSCs, AD-MSCs can significantly improve vessel formation and maintain the integrity of neovascular structure in an EPC +MSC/matrigel graft in SCID mice, especially under allo-PBMC induced immuno-rejection. In conclusion, our study shows that AD-MSC is a powerful candidate to minimize immunological rejection and improve vessel formation in EPC transplantation treatment.
引用
收藏
页数:15
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