Labeling of active neural circuits in vivo with designed calcium integrators

被引：325

作者：

Fosque, Benjamin F. ^{[1
]}

Sun, Yi ^{[1
]}

Dana, Hod ^{[1
]}

Yang, Chao-Tsung ^{[1
]}

Ohyama, Tomoko ^{[1
]}

Tadross, Michael R. ^{[1
]}

Patel, Ronak ^{[1
]}

Zlatic, Marta ^{[1
]}

Kim, Douglas S. ^{[1
]}

Ahrens, Misha B. ^{[1
]}

Jayaraman, Vivek ^{[1
]}

Looger, Loren L. ^{[1
]}

Schreiter, Eric R. ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Ashburn, VA 20147 USA

来源：

SCIENCE | 2015年 / 347卷 / 6223期

关键词：

NEURONAL-ACTIVITY; GENE-EXPRESSION; VISUAL-CORTEX; DROSOPHILA; BRAIN; PROTEIN; POPULATIONS; SENSOR; CELLS;

D O I：

10.1126/science.1260922

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The identification of active neurons and circuits in vivo is a fundamental challenge in understanding the neural basis of behavior. Genetically encoded calcium (Ca2+) indicators (GECIs) enable quantitative monitoring of cellular-resolution activity during behavior. However, such indicators require online monitoring within a limited field of view. Alternatively, post hoc staining of immediate early genes (IEGs) indicates highly active cells within the entire brain, albeit with poor temporal resolution. We designed a fluorescent sensor, CaMPARI, that combines the genetic targetability and quantitative link to neural activity of GECIs with the permanent, large-scale labeling of IEGs, allowing a temporally precise "activity snapshot" of a large tissue volume. CaMPARI undergoes efficient and irreversible green-to-red conversion only when elevated intracellular Ca2+ and experimenter-controlled illumination coincide. We demonstrate the utility of CaMPARI in freely moving larvae of zebrafish and flies, and in head-fixed mice and adult flies.

引用

页码：755 / 760

页数：6

共 32 条

[1] Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design [J].