LC-MS-MS Simultaneous Determination of l-Dopa and Its Prodrug l-Dopa n-Pentyl Ester Hydrochloride in Rat Plasma

被引：12

作者：

Lv, Li ^{[1
]}

Jiang, Weizhe ^{[1
]}

Zhou, Songyu ^{[1
]}

Huang, Xingzhen ^{[1
]}

Shi, Xiaoxia ^{[1
]}

Lv, Cong ^{[1
]}

Wu, Lingling ^{[1
]}

Xu, Chongyao ^{[1
]}

机构：

[1] Guangxi Med Univ, Sch Pharmaceut, Nanning 530021, Peoples R China

来源：

CHROMATOGRAPHIA | 2010年 / 72卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

Column liquid chromatography-tandem mass spectrometry; Pharmacokinetic study; L-Dopa; L-Dopa n-pentyl ester hydrochloride; PERFORMANCE LIQUID-CHROMATOGRAPHY; ELECTROCHEMICAL DETECTION; LEVODOPA; 3-O-METHYLDOPA; METABOLITES;

D O I：

10.1365/s10337-010-1668-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive, simple and rapid LC-MS-MS method has been developed and validated for the simultaneous determination of l-dopa and l-dopa n-pentyl ester hydrochloride in rat plasma in the present study. The analytes were separated on a C(18) column (5 mu m, 2.1 x 150 mm) with a security guard C(18) column (5 mu m, 4 x 20 mm) and a triple-quadrupole mass spectrometer was applied for detection. The method was linear over the concentration ranges of 25-5,000 ng mL(-1) for l-dopa and 12.5-2,500 ng mL(-1) for l-dopa n-pentyl ester hydrochloride. Finally, the method was successfully applied to support the pharmacokinetic study.

引用

页码：239 / 243

页数：5

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