Palladium-Mediated Synthesis of a Near-Infrared Fluorescent K+ Sensor

被引：14

作者：

Bandara, H. M. Dhamrnika ^{[1
]}

Hua, Zhengmao ^{[1
]}

Zhang, Mei ^{[1
]}

Pauff, Steven M. ^{[1
,3
]}

Miller, Stephen C. ^{[1
]}

Davie, Elizabeth A. Colby ^{[2
]}

Kobertz, William R. ^{[1
]}

机构：

[1] Univ Massachusetts, Med Sch, Dept Biochem & Mol Pharmacol, Programs Neurosci & Chem Biol, 364 Plantat St, Worcester, MA 01605 USA

[2] Assumption Coll, Dept Nat Sci, 500 Salisbury St, Worcester, MA 01609 USA

[3] Univ Strathclyde, Dept Pure & Appl Chem, 295 Cathedral St, Glasgow G1 1XL, Lanark, Scotland

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2017年 / 82卷 / 15期

基金：

美国国家卫生研究院;

关键词：

POTASSIUM ACCUMULATION; CALCIUM INDICATORS; CHANNELOPATHIES; SELECTIVITY; DYNAMICS; CHANNELS; MUSCLE; CORTEX; PROBE;

D O I：

10.1021/acs.joc.7b00845

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Potassium (K+) exits electrically excitable cells during normal and pathophysiological activity. Currently, K+-sensitive electrodes and electrical measurements are the primary tools to detect K+ fluxes. Here, we describe the synthesis of a near-IR, oxazine fluorescent K+ sensor (K-NIR-1) with a dissociation constant suited for detecting changes in intracellular and extracellular K+ concentrations. K-NIR-1 treatment of cells expressing voltage-gated K+ channels enabled the visualization of intracellular K+ depletion upon channel opening and restoration of cytoplasmic K+ after channel closing.

引用

页码：8199 / 8205

页数：7

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