Identification of putative miRNA biomarkers in early rheumatoid arthritis by genome-wide microarray profiling: A pilot study

被引:20
作者
Romo-Garcia, M. F. [1 ,7 ]
Bastian, Y. [1 ,2 ]
Zapata-Zuniga, M. [3 ,8 ]
Macias-Segura, N. [1 ,9 ]
Castillo-Ortiz, J. D. [4 ,5 ]
Lara-Ramirez, E. E. [1 ]
Fernandez-Ruiz, J. C. [1 ,7 ]
Berlanga-Taylor, A. J. [6 ]
Gonzalez-Amaro, R. [7 ]
Ramos-Remus, C. [4 ,5 ]
Enciso-Moreno, J. A. [1 ]
Castaneda-Delgado, J. E. [1 ,2 ]
机构
[1] IMSS, Unidad Invest Biomed Zacatecas, Zacatecas, Mexico
[2] Catedras CONACyT Unidad Invest Biomed Zacatecas I, Zacatecas, Mexico
[3] IMSS, Hosp Rural 51, Villanueva, Zacatecas, Mexico
[4] Unidad Invest Enfermedades Cren Degenerat, Guadalajara, Jalisco, Mexico
[5] Univ Autonoma Guadalajara, Guadalajara, Jalisco, Mexico
[6] Imperial Coll London, Fac Med, MRC PHE Ctr Environm & Hlth, Dept Epidemiol & Biostat,Sch Publ Hlth, St Marys Campus,Norfolk Pl, London, England
[7] Univ Autonoma San Luis Potosi, Fac Med, Dept Inmunol, San Luis Potosi, San Luis Potosi, Mexico
[8] Univ Autonoma Zacatecas, Fac Med Humana & Ciencias Salud, Zacatecas, Mexico
[9] Univ Autonoma Aguascalientes, Ctr Ciencias Basicas, Dept Fisiol & Farmacol, Lab Neuroinmunoendocrinol, Aguascalientes, Aguascalientes, Mexico
基金
英国医学研究理事会;
关键词
Early rheumatoid arthritis; Biomarker; miRNA; CCP; Microarray expression; CITRULLINATED PROTEIN ANTIBODIES; PLASMA MICRORNA; EXPRESSION; OSTEOARTHRITIS; DISEASE; RISK; SPONDYLOARTHROPATHY; POPULATIONS; ENVIRONMENT; DIAGNOSIS;
D O I
10.1016/j.gene.2019.144081
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Despite the existing research, the etiology of rheumatoid arthritis (RA), an autoimmune disease remains poorly understood with early and accurate diagnosis difficult to achieve. MicroRNAs (miRNAs) play an important role in biological processes as modulators of transcription and translation. Previous studies have demonstrated a downregulation of several genes in' early RA stages and in addition, miRNAs may serve as early biomarkers of subclinical changes in early RA. When comparing the four groups (ANOVA P < 0.01, fold change > 4), we found 253 differentially expressed miRNAs. Of these, 97 miRNAs were identified as overexpressed in early rheumatoid arthritis. The validation of miRNA microarray expression was performed in a set by RT-qPCR and showed strong agreement with microarray expression data. The putative targets of overexpressed microRNAs in early RA were significantly enriched in apoptosis, tolerance loss and Wnt pathways. Moreover, ROC analysis showed values of AUC 0.76 and P < 0.05 for miR 361-5p, identifying this miRNA as a potential biomarker of disease. We identified specific microRNAs associated with early rheumatoid arthritis and proposed them as early biomarkers of disease. Our results provide novel insight into immune disease physiopathology and describe unreported microRNAs in RA with potential for clinical use.
引用
收藏
页数:10
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