Structure of the O-specific polysaccharide of Proteus vulgaris O45 containing 3-acetamido-3,6-dideoxy-D-galactose

被引：7

作者：

Perepelov, AV ^{[1
]}

Bartodziejska, B

Senchenkova, SN

Shashkov, AS

Rozalski, A

Knirel, YA

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia

[2] Univ Lodz, Inst Microbiol & Immunol, Dept Bacterial Immunol, PL-90237 Lodz, Poland

来源：

CARBOHYDRATE RESEARCH | 2003年 / 338卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

Proteus vulgaris; lipopolysaccharide; O-antigen; bacterial polysaccharide structure; 3-acetamido-3,6-dideoxy-D-galactose;

D O I：

10.1016/S0008-6215(02)00361-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Proteus vulgaris O45 and studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, including 2D COSY, TOCSY, ROESY, H-detected H-1, C-13 HSQC and HMBC experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established: -->6)-alpha-D-GlcpNAc-(1-->4)-alpha-D-GalpNAc-(1-->4)-alpha-D-GalpA-(1-->3)-beta-D-GlcpNAc-(1-->2)-beta-D-Fucp3NAc4Ac-(1--> where Fuc3NAc4Ac is 3-acetamido-4-O-acetyl-3,6-dideoxygalactose. A cross-reactivity of anti-P. vulgaris O45 serum was observed with several other Proteus lipopolysaccharides, which contains Fuc3N derivatives. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：327 / 331

页数：5

共 21 条

[1] [Anonymous], 1972, METHODS CARBOHYDR CH
[2] Structural and serological studies on a new acidic O-specific polysaccharide of Proteus vulgaris O32
Bartodziejska, B
Shashkov, AS
Babicka, D
Grachev, AA
Torzewska, A
Paramonov, NA
Chernyak, AY
Rozalski, A
Knirel, YA
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 256 (02): : 488 - 493
[3] Structure and serological specificity of a new acidic O-specific polysaccharide of Proteus vulgaris O45
Bartodziejska, B
Shashkov, AS
Torzewska, A
Grachev, AA
Ziolkowski, A
Paramonov, NA
Rozalski, A
Knirel, YA
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 259 (1-2): : 212 - 217
[4] C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF MONOSACCHARIDES
BOCK, K
PEDERSEN, C
[J]. ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1983, 41 : 27 - 66
[5] DRZEWIECKA D, 2002, IN PRESS CARBOHYDR R
[6] GERWIG GJ, 1979, CARBOHYD RES, V77, P1, DOI 10.1016/S0008-6215(00)83788-X
[7] STRUCTURAL STUDIES OF THE PUTATIVE O-SPECIFIC POLYSACCHARIDE OF ACINETOBACTER-BAUMANNII O2 CONTAINING 3,6-DIDEOXY-3-N-(D-3-HYDROXYBUTYRYL)AMINO-D-GALACTOSE
HASELEY, SR
WILKINSON, SG
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 233 (03): : 899 - 906
[8] COMPUTER-ASSISTED STRUCTURAL-ANALYSIS OF POLYSACCHARIDES WITH AN EXTENDED VERSION OF CASPER USING H-1-NMR AND C-13-NMR DATA
JANSSON, PE
KENNE, L
WIDMALM, G
[J]. CARBOHYDRATE RESEARCH, 1989, 188 : 169 - 191
[9] NUCLEAR-MAGNETIC-RESONANCE AND CONFORMATIONAL STUDIES ON MONOACETYLATED METHYL D-GLUCO-PYRANOSIDES AND D-GALACTO-PYRANOSIDES
JANSSON, PE
KENNE, L
SCHWEDA, E
[J]. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1987, (02): : 377 - 383
[10] Knirel YA, 1999, POL J CHEM, V73, P895

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