Investigation of pathogenesis of hyperuricemia based on untargeted and targeted metabolomics

被引：22
作者：
Qin, Nankun [1 ]
Qin, Ming [1 ]
Shi, Wenjun [1 ]
Kong, Lingbo [2 ]
Wang, Liting [1 ]
Xu, Guang [1 ]
Guo, Yuying [1 ]
Zhang, Jiayu [3 ]
Ma, Qun [1 ]
机构：
[1] Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 102488, Peoples R China
[2] Beijing Univ Chinese Med, Affiliated Dongzhimen Hosp, Beijing 100010, Peoples R China
[3] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
来源：
SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期
关键词：
MASS-SPECTROMETRY; PALMITIC ACID; METABOLISM;
D O I：
10.1038/s41598-022-18361-y
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Hyperuricemia (HUA) seriously harms human health but the exact etiology and pathogenesis of HUA are not fully understood. Therefore, it is still of great significance to find effective biomarkers and explore the pathogenesis of HUA. Metabolomics reflects the influence of internal and external factors on system metabolism, explains the changes in metabolite levels during the development of diseases, and reveals the molecular mechanism of pathogenesis. Metabolomics is divided into untargeted metabolomics and targeted metabolomics according to different research modes. Each other's advantages can be fully utilized by combining the two so that the results of metabolomics research can be consummated. 20 HUA patients and 20 healthy individuals participated in the experiment, and untargeted metabolomics was employed to find 50 differential metabolites in HUA serum samples. Twelve candidate biomarkers were screened based on literature research and ROC Curve analysis for subsequent verification. Based on the UPLC-TQ-MS analysis platform, the targeted metabolomics detection methods were established and the content of 12 candidate biomarkers was precisely quantified. Compare with the results of untargeted metabolomics, the targeted metabolomics results were considered more reliable.
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页数：14
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