Dual-sided illumination light-sheet fluorescence microscopy with virtual single-pixel imaging deconvolution

被引：1

作者：

Hu Jin-Hu ^{[1
]}

Lin Dan-Ying ^{[1
]}

Zhang Wei ^{[2
]}

Zhang Chen-Shuang ^{[1
]}

Qu Jun-Le ^{[1
]}

Yu Bin ^{[1
]}

机构：

[1] Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Shenzhen 518060, Peoples R China

[2] Foshan Polytech, Coll Electromech Engn, Foshan 528137, Peoples R China

来源：

ACTA PHYSICA SINICA | 2022年 / 71卷 / 02期

基金：

中国国家自然科学基金;

关键词：

light-sheet fluorescence microscopy; virtual single-pixel imaging; deconvolution; three-dimensional imaging; RECONSTRUCTION; RESOLUTION; DYNAMICS; DEEP;

D O I：

10.7498/aps.71.20211358

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

In light-sheet fluorescence microscopy (LSFM) a thin light sheet is used to excite the specimen from the side and imaging is performed in the direction perpendicular to the light-sheet. It has the advantages of fast imaging speed, high optical sectioning capability and low photobleaching and phototoxicity to samples. Therefore, it is suitable for high-quality, long-term three-dimensional dynamic observation of large living biological samples. However, the traditional Gaussian light sheet illumination microscopy technology has the problems of small imaging field of view and low spatial resolution. Based on the existing dual-sided illumination LSFM, a large field of view and high resolution LSFM combined with virtual single-pixel imaging deconvolution is presented in this paper, which improves the field of view and resolution of LSFM simultaneously. The relevant microscope is designed and built, and three-dimensional optical sectioning imaging experiments on fluorescent beads and transgenic zebrafish standard samples are carried out. The experimental results prove the three-dimensional high resolution imaging capability of the microscope, which is of great significance in developing the large field of view and high resolution LSFM.

引用

页数：7

共 25 条

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