Structure and stress-related expression of two cDNAs encoding proteinase inhibitor II of Nicotiana glutinosa L.

被引:32
作者
Choi, D
Park, JA
Seo, YS
Chun, YJ
Kim, WT [1 ]
机构
[1] Yonsei Univ, Coll Sci, Dept Biol, Seoul 120749, South Korea
[2] Korea Res Inst Biosci & Biotechnol, Plant Cell Biotechnol Lab, Taejeon 305600, South Korea
[3] Yonsei Univ, Coll Sci, Inst Life Sci, Seoul 120749, South Korea
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1492卷 / 01期
关键词
cDNA; proteinase inhibitor; stress-induced gene expression; tissue-specific expression; tobacco mosaic virus; Nicotiana glutinosa L;
D O I
10.1016/S0167-4781(00)00073-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54-82% identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:211 / 215
页数:5
相关论文
共 18 条
  • [1] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [2] PROTEINASE-INHIBITORS IN NICOTIANA-ALATA STIGMAS ARE DERIVED FROM A PRECURSOR PROTEIN WHICH IS PROCESSED INTO 5 HOMOLOGOUS INHIBITORS
    ATKINSON, AH
    HEATH, RL
    SIMPSON, RJ
    CLARKE, AE
    ANDERSON, MA
    [J]. PLANT CELL, 1993, 5 (02) : 203 - 213
  • [3] STRUCTURE AND INDUCTION-PATTERN OF A NOVEL PROTEINASE-INHIBITOR CLASS-II GENE OF TOBACCO
    BALANDIN, T
    VANDERDOES, C
    ALBERT, JMB
    BOL, JF
    LINTHORST, HJM
    [J]. PLANT MOLECULAR BIOLOGY, 1995, 27 (06) : 1197 - 1204
  • [4] Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals
    Bergey, DR
    Howe, GA
    Ryan, CA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (22) : 12053 - 12058
  • [5] Molecular cloning of a metallothionein-like gene from Nicotiana glutinosa L and its induction by wounding and tobacco mosaic virus infection
    Choi, D
    Kim, HM
    Yun, HK
    Park, JA
    Kim, WT
    Bok, SH
    [J]. PLANT PHYSIOLOGY, 1996, 112 (01) : 353 - 359
  • [6] GRAHAM JS, 1985, J BIOL CHEM, V260, P6561
  • [7] PROGRAMMED CELL-DEATH IN PLANTS - A PATHOGEN-TRIGGERED RESPONSE ACTIVATED COORDINATELY WITH MULTIPLE DEFENSE FUNCTIONS
    GREENBERG, JT
    GUO, AL
    KLESSIG, DF
    AUSUBEL, FM
    [J]. CELL, 1994, 77 (04) : 551 - 563
  • [8] HEATH RL, 1995, EUR J BIOCHEM, V230, P250, DOI 10.1111/j.1432-1033.1995.tb20558.x
  • [9] PRIMARY STRUCTURE OF A PROTEINASE INHIBITOR-II GENE FROM POTATO (SOLANUM-TUBEROSUM)
    KEIL, M
    SANCHEZSERRANO, J
    SCHELL, J
    WILLMITZER, L
    [J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (14) : 5641 - 5650
  • [10] Biotic and abiotic stress-related expression of 1-aminocyclopropane-1-carboxylate oxidase gene family in Nicotiana glutinosa L.
    Kim, YS
    Choi, D
    Lee, MM
    Lee, SH
    Kim, WT
    [J]. PLANT AND CELL PHYSIOLOGY, 1998, 39 (06) : 565 - 573