Nanosafety evaluation through feces: A comparison between selenium nanoparticles and selenite in rats

被引：31

作者：

Lin, Xiaoying ^{[1
,2
]}

Wang, Liming ^{[2
,3
]}

Zhao, Jiating ^{[2
,3
]}

He, Lina ^{[2
]}

Cui, Liwei ^{[3
]}

Gao, Yuxi ^{[2
,3
]}

Chen, Chunying ^{[3
,4
,5
,6
]}

Fan, Yuqin ^{[7
]}

Li, Bai ^{[2
]}

Li, Yu-Feng ^{[2
,3
]}

机构：

[1] Jilin Med Univ, Jilin 132013, Jilin, Peoples R China

[2] Chinese Acad Sci, Inst High Energy Phys, Natl Consortium Excellence Metall,CAS Key Lab Bio, CAS HKU Joint Lab Metall Hlth & Environm,Beijing, Beijing 100049, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[4] Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100191, Peoples R China

[5] Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, Beijing 100191, Peoples R China

[6] Natl Ctr Nanosci & Technol, Beijing Metall Facil, Beijing 100191, Peoples R China

[7] Shandong Univ, Shandong Prov Maternal & Child Hlth Care Hosp, Cheeloo Coll Med, Jinan 250014, Shandong, Peoples R China

来源：

NANO TODAY | 2021年 / 36卷

基金：

中国国家自然科学基金;

关键词：

Se(0)NPs; Feces; Intestinal microbiota; Metabolites; Metallomics;

D O I：

10.1016/j.nantod.2020.101010

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanosafety evaluation is necessary not only for nanomaterials research and development but also as a cornerstone for legal regulation. In this study, we proposed a non-invasive protocol for nanosafety evaluation through feces using metagenomics and metabolomics together with metallomics. Male SD rats were orally exposed to equal amounts of Se (Se(0)NPs, 3.14 or Na2SeO3, 6.28 mu g/kg bw) and were sacrificed after 24 h. 16S rRNA analysis and LC-MS were used to study the impact of different forms of Se on intestinal microbiota and metabolites in fecal samples. ICP-MS and synchrotron radiation X-ray fluorescence (SR-XRF) and X-ray absorption spectroscopy (XAS) were used to study the concentration and transformation of Se in gastrointestinal track and feces. It was found that Se NPs brought less alternation to the composition of intestinal microbiota and metabolites related to inflammation, immunity and gut-brain axis related responses than Na2SeO3 did. Besides, the absorbed Se(0)NPs could be more efficiently converted to organic Se which explained the comparable bioavailability to Na2SeO3. In all, the proposed protocol combined with metagenomics, metabolomics and metallomics in this study can be used for nanosafety evaluation in a non-invasive manner and this may also be used for the screening of the nanosafety of other nanomaterials. (C) 2020 Elsevier Ltd. All rights reserved.

引用

页数：8

共 51 条

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