Revisiting the Conformation and Dynamics of DNA in Slitlike Confinement

被引：116

作者：

Tang, Jing ^{[1
]}

Levy, Stephen L. ^{[2
]}

Trahan, Daniel W. ^{[1
]}

Jones, Jeremy J. ^{[1
]}

Craighead, Harold G. ^{[2
]}

Doyle, Patrick S. ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY 14853 USA

来源：

MACROMOLECULES | 2010年 / 43卷 / 17期

基金：

美国国家科学基金会;

关键词：

DOUBLE-STRANDED DNA; POLYMER-CHAINS; NANOSLITS; DIFFUSION; FLOW; MACROMOLECULES; STATISTICS; MOLECULES; TRANSPORT; PLATES;

D O I：

10.1021/ma101157x

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

We experimentally investigated the equilibrium conformation and dynamics of single DNA molecules in slitlike nanochannels. We measured the in-plane radius of gyration (R(parallel to)), diffusivity (D), and longest relaxation time (tau) of lambda-DNA (48.5 kbp) as functions of the slit height using fluorescence microscopy. Our results show that the in-plane radius of gyration increases monotonically with decreasing slit height, in contrast to results from Bonthuis et al. (15)but in agreement with our simulations and those of other groups. In strong confinement (slit height <100 nm), the scaling of D, tau, and R(parallel to) with slit height does not show an evident change, suggesting that the transition from the de Gennes regime to the Odijk regime is gradual and broad.

引用

页码：7368 / 7377

页数：10

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