Serological evidence of hepatitis E virus infection in dromedary camels in Ethiopia

被引：19

作者：

Li, Tian-Cheng ^{[1
]}

Yoshizaki, Sayaka ^{[1
]}

Zhou, Xianfeng ^{[2
]}

Sentsui, Hiroshi ^{[3
]}

Shirato, Kazuya ^{[4
]}

Matsuyama, Shutoku ^{[4
]}

Melaku, Simenew Keskes ^{[5
]}

Bazartseren, Boldbaatar ^{[6
]}

Takeda, Naokazu ^{[7
]}

Wakita, Takaji ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Virol 2, Gakuen 4-7-1, Tokyo 2080011, Japan

[2] Nanchang Ctr Dis Control & Prevent, Dept Microbiol, Nanchang, Jiangxi, Peoples R China

[3] Nihon Univ, Dept Vet Med, 1866 Kameino, Fujisawa, Kanagawa 2520880, Japan

[4] Natl Inst Infect Dis, Dept Virol 3, Gakuen 4-7-1, Tokyo 2080011, Japan

[5] Addis Ababa Sci & Technol Univ, Coll Biol & Chem Engn, Addis Ababa, Ethiopia

[6] Inst Vet Med, Zaisan 17024, Ulaanbaatar, Mongolia

[7] Osaka Univ, Res Inst Microbial Dis, Suita, Osaka 5650781, Japan

来源：

JOURNAL OF VIROLOGICAL METHODS | 2017年 / 246卷

关键词：

Dromedary camel HEV; DcHEV; ELISA; Anti-DcHEV IgG;

D O I：

10.1016/j.jviromet.2017.04.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The genome of dromedary camel hepatitis E virus (DcHEV) has been detected in stool and serum samples from dromedary camels, but the sero-epidemiological information of DcHEV infection remains unclear. A total of 246 serum samples collected from dromedary camels (Camelus dromedarius) in Ethiopia, and 40 serum samples from Bactrian camels (Camelus ferns) in Mongolia were examined for the detection of anti-DcHEV IgG antibody by a newly developed enzyme-linked immunosorbent assay (ELISA) by using DcHEV-like particles (DcHEV-LPs) as the antigen. The results revealed that 55 of the 246 (22.4%) dromedary camels were positive for anti-DcHEV IgG, whereas all 40 samples from the Bactrian camels were negative for DcHEV IgG antibody. A total of 98 serum samples from dromedary camels, including 25 anti-DcHEV-IgG positive samples, were used for the detection of DcHEV RNA by reverse transcription-polymerase chain reaction (RT-PCR), however, no positive samples were identified. These results suggested that the DcHEV infection occurred in the dromedary camels in Ethiopia. Further studies are required to determine whether Bactrian camels are susceptible to DcHEV infection. In addition, not only DcHEV-LPs, but also virus-like particles (VLPs) delivered from Gl, G3 and G5 HEV are likely applicable for the detection of the anti-DcHEV IgG antibody.

引用

页码：34 / 37

页数：4

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